Volume 17 Issue 2
Apr.  2021
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ZHANG Wenwei, HUANG Zhong, DUAN Yafei, LIN Heizhao, JIANG Shigui, HUANG Jianhua. Effects of different disinfectants on water quality and microbial community structure of Litopenaeus vannamei[J]. South China Fisheries Science, 2021, 17(2): 1-10. DOI: 10.12131/20200236
Citation: ZHANG Wenwei, HUANG Zhong, DUAN Yafei, LIN Heizhao, JIANG Shigui, HUANG Jianhua. Effects of different disinfectants on water quality and microbial community structure of Litopenaeus vannamei[J]. South China Fisheries Science, 2021, 17(2): 1-10. DOI: 10.12131/20200236
shu

Effects of different disinfectants on water quality and microbial community structure of Litopenaeus vannamei

  • 1.

    College of Life Science, Guangzhou University, Guangzhou 510006, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

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  • Received Date: November 13, 2020
  • Revised Date: December 27, 2020
  • Available Online: January 19, 2021
    Graphical Abstract
    • Abstract
  • We analyzed the total number of heterotrophic bacteria and Vibrio, ammonia and nitrite nitrogen of water, survival rate of larvae, bacterial community of larvae and rearing water of Litopenaeus vannamei after different disinfection treatment of hatchery water, including iodine (I2), chlorine dioxide solution (ClO2), formaldehyde (HCHO) and bleach [Ca(ClO)2]. The results show that the total number of bacteria and Vibrio in the formaldehyde group was lower, and the number of Vibrio was the lowest. The concentration of ammonia and nitrite nitrogen in the four disinfectants groups was lower than that in the control group, and the concentration of bleach group was the lowest. The survival rate of the larvae in the formaldehyde group was significantly higher than those in the other four groups (P<0.05). The 16S rDNA high-throughput sequencing reveals that the disinfectant changed the microbial community and abundance of dominant bacteria of the hatchery water and the larvae. The bacterial composition of the hatchery water was more complex than that of the larvae, and the early stage was more complex than the late stage. Besides, the genera OM43_clade, Alcanivorax, Erythrobacter, Altererythrobacter, Tenacibaculum, Enhydrobacter and Gilvibacter were enriched in the N6−Z1 periods of rearing water; Donghicola, Cohaesibacter, Phaeodactylibacter, Candidatus-Cquiluna and Ruegeria were enriched in the M1−P1 periods of rearing water, while Vibrio, Planomicrobium, Exiguobacterium, Psychrobacter, Acinetobacter, Bacillus, Methylophaga and Halomonas had high abundance in the larvae.
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