Volume 17 Issue 1
Feb.  2021
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LÜ Jiatong, LIN Haisheng, QIN Xiaoming, ZHANG Chaohua, CAO Wenhong, GAO Jialong, ZHENG Huina. Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging[J]. South China Fisheries Science, 2021, 17(1): 91-100. DOI: 10.12131/20200141
Citation: LÜ Jiatong, LIN Haisheng, QIN Xiaoming, ZHANG Chaohua, CAO Wenhong, GAO Jialong, ZHENG Huina. Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging[J]. South China Fisheries Science, 2021, 17(1): 91-100. DOI: 10.12131/20200141
shu

Preliminary study on resistance of oyster and its enzymatic hydrolysis products to skin photoaging

  • 1.

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China

  • 2.

    Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety/Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution/National Research and Development Branch Center for Shellfish Processing (Zhanjiang)/South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China

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  • Received Date: July 06, 2020
  • Revised Date: August 20, 2020
  • Accepted Date: October 15, 2020
  • Available Online: December 01, 2020
    Graphical Abstract
    • Abstract

  • To explore the protective effects of Hong Kong oyster (Crassostrea hongkongensis) meat (OM) and its enzymatic hydrolysis (OH) products on UV-induced photoaging of mouse (Mus musculus) skin. Ultraviolet rays (UVA+UVB) were irradiated every day to build a mouse skin photoaging model. The mice were given OM and OH daily and divided into low, medium and high dose groups (30, 90, 180 mg·kg−1) for eight weeks. The results show that OM and OH can reduce mice skin wrinkles, improve skin elasticity, reduce subcutaneous blood vessel atrophy, and prevent increase in vascular permeability. The results of skin HE staining show that medium and high doses of OM and OH can slow down hyperkeratosis and epidermis thickening significantly (P<0.05). Masson staining and aldehyde fuchsin staining results show that OM and OH can reduce the curling and degradation of collagen fibers, avoid abnormal proliferation of elastic fibers, and restore the arrangement of the dermal extracellular matrix network. Compared with the model group, OM and OH can increase the enzyme activity of superoxide dismutase (SOD) and glutathione catalase (GSH-Px) in skin, while malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHDG) concentration decreased significantly (P<0.05). OM and OH can inhibit the expression of matrix metalloproteinases (MMP-3, MMP-9), and reduce the degradation of hydroxyproline (Hyp) in the dermis significantly. In addition, high doses of OH can reduce the expression of inflammatory factors, interleukin 6 (IL-6) and tumor necrosis factor (TNF-α) in the skin significantly, and increase the skin's anti-inflammatory ability by increasing transforming growth factor (TGF-β).

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