Volume 16 Issue 4
Aug.  2020
Turn off MathJax
Article Contents
Abstract
References
Related Articles
CHEN Zicong, CHEN Pimao, YUAN Huarong, FENG Xue, TONG Fei, ZHANG Haoming. Study on respiratory metabolism changes of juvenile Penaeus monodon following strenuous activity[J]. South China Fisheries Science, 2020, 16(4): 75-83. DOI: 10.12131/20200017
Citation: CHEN Zicong, CHEN Pimao, YUAN Huarong, FENG Xue, TONG Fei, ZHANG Haoming. Study on respiratory metabolism changes of juvenile Penaeus monodon following strenuous activity[J]. South China Fisheries Science, 2020, 16(4): 75-83. DOI: 10.12131/20200017
shu

Study on respiratory metabolism changes of juvenile Penaeus monodon following strenuous activity

  • 1.

    College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of South China Sea Fishery Resources and Environment, Ministry of Agriculture and Rural Affairs/Key Laboratory of Marine Ranching Technology, Chinese Academy of Fishery Sciences/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment/Guangdong Engineering Technology Research Center of Marine Recreational Fishery

More Information
  • Received Date: February 08, 2020
  • Revised Date: March 14, 2020
  • Accepted Date: March 17, 2020
  • Available Online: March 26, 2020
    Graphical Abstract
    • Abstract

  • In order to provide scientific basis for the breeding and releasing of Penaeus monodon, we simulated the exhausted movement of the juveniles in the laboratory flume, then investigated their respiratory metabolic recovery after exhaustion exercise. With different recovery times after exhaustive exercise, there were significant changes in the total protein content (TP) and lactic acid (LA) concentrations as well as the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in the hepatopancreas of the juveniles (P<0.05). There was significant difference in the LA concentration and LDH activity in abdominal muscles. The TP concentration in hepatopancreas was significantly higher than that of the control group after 4 h of exhaustive exercise, and returned to the control level at 48th hour. The LA concentration of hepatopancreas was significantly higher than that of the control group at 12th hour, and returned to the control level at 24th hour. The activities of AST and ALT in hepatopancreas increased significantly during the 8th to 12th hour, and returned to the control level at 24th hour. The activities of LDH and SDH increased and were significantly higher than that of the control group at  12th hour, and returned to the control level at 48th hour. The glycogen concentration (GLY) in the muscle decreased significantly after exhaustive exercise and returned to the control level at the 12th hour. The LA concentration increased significantly after exhaustive exercise and decreased to the control level at 8th hour. The LDH activity increased significantly after exhaustive exercise, and continued to increase during 1st to 12th hour, and remained significantly higher than that of the control group at 72th hour. The anaerobic metabolism of the juveniles was significantly enhanced after exhaustive exercise, and exhaustive exercise could be performed again after 8 h; heaptopancreas was the place where the juveniles cleared metabolites, and began to deal with internal environment imbalance after exercise.

    • FullText(HTML)
    • References (40)
  • [1]
    KIEFFER J D. Limits to exhaustive exercise in fish[J]. Comp Biochemis Physiol A, 2000, 126(2): 161-179. doi: 10.1016/S1095-6433(00)00202-6
    [2]
    KIEFFER J D. Perspective-Exercise in fish: 50+years and going strong[J]. Comp Biochem Physiol A, 2010, 156(2): 163-168. doi: 10.1016/j.cbpa.2010.02.009
    [3]
    GREGORYM A. A comparison of startle response in two morphs of the brook stickleback (Culaea inconstans): further evidence for a trade-off between defensive morphology and swimming ability[J]. Evol Ecol, 1997, 11(1): 83-90. doi: 10.1023/A:1018487529938
    [4]
    DALL W, HILL B J, ROTHLISBERG P C, et al. The biology of the Penaeidae[J]. J Crus Biol, 1990, 27(4): 325-329.
    [5]
    张沛东. 对虾行为生理生态学的实验研究[D]. 青岛: 中国海洋大学, 2006: 4-5.
    [6]
    唐小红, 樊佳佳, 于凌云, 等. 鱼类糖酵解关键酶的研究进展[J]. 中国农学通报, 2014(2): 69-75.
    [7]
    朱晏苹, 曹振东, 付世建. 力竭性运动后瓦氏黄颡鱼幼鱼乳酸和血糖变化[J]. 重庆师范大学学报(自然科学版), 2010, 27(2): 14-17.
    [8]
    李秀明, 张耀光, 何春梅, 等. 力竭追赶训练对两种鲤科鱼类呼吸循环系统参数和力竭运动后代谢特征的影响[J]. 水生生物学报, 2019, 43(1): 78-85.
    [9]
    苗增良, 李鹏, 周秀锦, 等. 急性力竭性运动后日本黄姑鱼乳酸、糖原和葡萄糖水平变化[J]. 江苏农业科学, 2012, 40(9): 212-214. doi: 10.3969/j.issn.1002-1302.2012.09.086
    [10]
    李可贵. 摄食、力竭性运动及其交互作用对鲇鱼幼鱼代谢及酸碱状态的影响[M]. 重庆: 重庆师范大学, 2010: 17-23.
    [11]
    ZHANG Y, TIMMERHAUS G, ANTTILA K, et al. Domestication compromises athleticism and respiratory plasticity in response to aerobic exercise training in Atlantic salmon (Salmo salar)[J]. Aquaculture, 2016, 463: 79-88. doi: 10.1016/j.aquaculture.2016.05.015
    [12]
    SKJERVOLD P O, FJÆRA S O, ØSTBY P B, et al. Live-chilling and crowding stress before slaughter of Atlantic salmon (Salmo salar)[J]. Aquaculture, 2001, 192(2): 265-280.
    [13]
    李丹丹, 陈丕茂, 朱爱意, 等. 黑鲷幼鱼力竭运动后代谢酶活性的恢复水平[J]. 南方水产科学, 2018, 14(6): 59-65. doi: 10.12131/20180064
    [14]
    刘明镜, 于丽娟, 皇甫加清, 等. 无氧运动诱导的斑马鱼代谢及适应性变化[J]. 水生生物学报, 2013, 37(6): 1153-1157.
    [15]
    于晓明. 三种对虾游泳能力及其游泳生理的比较实验研究[D]. 青岛: 中国海洋大学, 2009: 26-55.
    [16]
    ZHANG P, ZHANG X, LI J, et al. Swimming ability and physiological response to swimming fatigue in whiteleg shrimp, Litopenaeus vannamei[J]. Comp Biochem Physiol A, 2006, 145(1): 26-32. doi: 10.1016/j.cbpa.2006.04.014
    [17]
    陈国福, 黄倢, 宋晓玲. 对虾免疫机能研究概况[J]. 水产学报, 2004, 28(2): 209-215.
    [18]
    郭彪, 王芳, 侯纯强, 等. 温度突变对凡纳滨对虾己糖激酶和丙酮酸激酶活力以及热休克蛋白表达的影响[J]. 中国水产科学, 2008, 15(5): 885-889. doi: 10.3321/j.issn:1005-8737.2008.05.024
    [19]
    张华军, 李卓佳, 张家松, 等. 密度胁迫对凡纳滨对虾稚虾免疫指标及生长的影响[J]. 南方水产科学, 2012, 8(4): 43-48. doi: 10.3969/j.issn.2095-0780.2012.04.007
    [20]
    张海恩, 何玉英, 李健, 等. 密度胁迫对中国对虾幼虾生长、抗氧化系统功能及水质指标的影响[J]. 渔业科学进展, 2020, 41(2): 140-149. doi: 10.19663/j.issn2095-9869.20190122001
    [21]
    段妍. 凡纳滨对虾游泳行为生理生态学实验研究[D]. 青岛: 中国海洋大学, 2012: 21-40.
    [22]
    丁阳阳, 江世贵, 李运东, 等. 斑节对虾Pellino基因的克隆及其在不同胁迫条件下的表达分析[J]. 南方水产科学, 2019, 15(3): 87-96. doi: 10.12131/20180216
    [23]
    MILLIGAN C L, HOOKE G B, JOHNSON C. Sustained swimming at low velocity following a bout of exhaustive exercise enhances metabolic recovery in rainbow trout[J]. J Exp Biol, 2000, 203(5): 921-926.
    [24]
    李黎, 曹振东, 付世建. 力竭性运动后鲇鱼幼鱼乳酸、糖原和葡萄糖水平的变动[J]. 水生生物学报, 2007, 31(6): 880-885. doi: 10.3321/j.issn:1000-3207.2007.06.017
    [25]
    朱晏苹, 曹振东, 付世建. 不同游泳速度条件下瓦氏黄颡幼鱼的有氧和无氧代谢反应[J]. 水生生物学报, 2010, 34(5): 905-912.
    [26]
    LIU Y, CAO Z D, FU S J, et al. The effect of exhaustive chasing training and detraining on swimming performance in juvenile dark barbel catfish (Peltebagrus vachelli)[J]. J Comp Physiol B, 2009, 179(7): 847-855. doi: 10.1007/s00360-009-0365-1
    [27]
    李根瑞. 仿刺参对环境低氧胁迫的生理生化响应研究[M]. 上海: 上海海洋大学, 2016: 27-33.
    [28]
    管越强, 李利, 王慧春, 等. 低氧胁迫对日本沼虾呼吸代谢和抗氧化能力的影响[J]. 河北大学学报 (自然科学版), 2010, 30(3): 301-306.
    [29]
    BROOKS, GEORGE A. The lactate shuttle during exercise and recovery[J]. Medic Sci Spor Exer, 1986, 18(3): 360-368. doi: 10.1249/00005768-198606000-00019
    [30]
    ZEHRA S, KHAN M A. Dietary pyridoxine requirement of fingerling Channa punctatus (Bloch) based on growth performance, liver pyridoxine concentration, and carcass composition[J]. J Appl Aquacult, 2018, 30(3): 238-255. doi: 10.1080/10454438.2018.1456999
    [31]
    周莹, 王际英, 李宝山, 等. 许氏平鲉幼鱼对饲料中维生素B6需求量的研究[J]. 渔业科学进展, 2019: 1-14. https://doi.org/10.19663/j.issn2095-9869.20191012001.
    [32]
    黄凯, 吴宏玉, 蒋焕超, 等. 饥饿对凡纳滨对虾能量代谢及脂肪酸组分的影响[J]. 水生态学杂志, 2011, 32(1): 105-110.
    [33]
    CHENG W, LIU C, KUO C. Effects of dissolved oxygen on hemolymph parameters of freshwater giant prawn, Macrobrachium rosenbergii (de Man)[J]. Aquaculture, 2003, 220(1/2/3/4): 843-856.
    [34]
    查蒙, 管维良, 茅林春. 山梨醇对凡纳滨对虾无水运输中呼吸和能量代谢的影响[J]. 食品研究与开发, 2019, 40(1): 30-36. doi: 10.3969/j.issn.1005-6521.2019.01.006
    [35]
    MOOSAVI B, BERRY E A, ZHU X, et al. The assembly of succinate dehydrogenase: a key enzyme in bioenergetics[J]. Cel Mol Life Sci, 2019, 76(20): 4023-4042. doi: 10.1007/s00018-019-03200-7
    [36]
    王兴强, 曹梅, 马甡, 等. 密度对凡纳滨对虾存活、生长和能量收支的影响[J]. 中国农学通报, 2005(8): 409-412. doi: 10.3969/j.issn.1000-6850.2005.08.115
    [37]
    ANAND P S, BALASUBRAMANIAN C P, CHRISTINA L, et al. Substrate based black tiger shrimp, Penaeus monodon culture: stocking density, aeration and their effect on growth performance, water quality and periphyton development[J]. Aquaculture, 2019, 507: 411-418. doi: 10.1016/j.aquaculture.2019.04.031
    [38]
    FRANKLIN C E, JOHNSTON I A, BATTY R S, et al. Metabolic recovery in herring larvae following strenuous activity[J]. J Fish Biol, 1996, 48(2): 207-216. doi: 10.1111/j.1095-8649.1996.tb01113.x
    [39]
    SAVANT K B, AMTE G K. Influence of some environmental-factors on respiratory responses in the tropical estuarine crab Ilyoplax gangetica (Kemp)[J]. J Environ Biol, 1995, 16(4): 311-317.
    [40]
    陶易凡, 强俊, 王辉, 等. 低pH胁迫对克氏原螯虾鳃和肝胰腺酶活力及组织结构的影响[J]. 中国水产科学, 2016, 23(6): 1279-1289.
    • Related Articles

  • Related Articles

    [1]YU Weihuan, TU Youkai, WU Jinhui, YOU Lei, YU Zonghe. Embryonic and larval development of sea urchin (Temnopleurus reevesii)[J]. South China Fisheries Science. DOI: 10.12131/20240241
    [2]ZHENG Xing, LIN Siqi, YANG Shouguo, ZHANG Xingzhi, VASQUEZ Herbert Ely, GU Zhifeng, WANG Aimin. Change and correlation analysis of pigment contents and color value during growth of Chlorella vulgaris[J]. South China Fisheries Science, 2021, 17(1): 32-38. DOI: 10.12131/20200076
    [3]LI Dandan, CHEN Pimao, ZHU Aiyi, YUAN Huarong, CHEN Wenjing, LONG Xinling, WANG Wenjie. Recovery level of metabolic enzymes in juvenile black sea bream (Sparus macrocephlus) after exhaustive exercise[J]. South China Fisheries Science, 2018, 14(6): 59-65. DOI: 10.12131/20180064
    [4]YUAN Yongming, SHI Pei. Study on fish movement monitoring method based on image processing[J]. South China Fisheries Science, 2018, 14(5): 109-114. DOI: 10.3969/j.issn.2095-0780.2018.05.014
    [5]HAO Shuxian, HE Dan, WEI Ya, LI Laihao, YANG Xianqing, HUANG Hui, LIN Wanling. Research progress in types of fish egg processing products and caviar preservation[J]. South China Fisheries Science, 2014, 10(3): 104-108. DOI: 10.3969/j.issn.2095-0780.2014.03.016
    [6]XU Wenqi, SHEN Jian. Research progress in shellfish pre-processing techniques in China[J]. South China Fisheries Science, 2013, 9(2): 76-80. DOI: 10.3969/j.issn.2095-0780.2013.02.013
    [7]WEI Ya, WU Yanyan, LI Laihao, YANG Xianqing, CHEN Shengjun, DIAO Shiqiang, HUANG Hui, ZHOU Wanjun, GONG Xiaojing. Quality and safety management in on-board processing of Engraulis japonius[J]. South China Fisheries Science, 2011, 7(2): 61-67. DOI: 10.3969/j.issn.2095-0780.2011.02.010
    [8]LIANG Ying, JIN Yue-mei, TIAN Chuan-yuan. Effects of phosphorus restriction and supplement on the chlorophyll fluorescent parameters of Chlorella sp.[J]. South China Fisheries Science, 2008, 4(4): 1-7.
    [9]ZHOU Wanjun, WU Yanyan, LI Laihao, XU Zezhi, YANG Xianqing, CHEN Shengjun, DIAO Shiqiang, CEN Jianwei. Study on the processing technology of instant fried fish skin[J]. South China Fisheries Science, 2006, 2(1): 62-65.
    [10]YANG Xian-qing, LI Lai-hao, WU Yan-yan, DIAO Shi-qiang, LI Liu-dong, CHEN Pei-ji, HAO Shu-xian. Studies on the processing technology and formula of ready-to-eat jellyfish[J]. South China Fisheries Science, 2005, 1(2): 46-50.

  • Cited by

    Periodical cited type(1)

    1. 高杰，郭华阳，刘明鉴，刘宝锁，朱克诚，张楠，郭梁，张殿昌. 无乳链球菌感染对卵形鲳鲹脾脏的损伤及细胞凋亡相关基因表达的影响. 南方水产科学. 2022(06): 44-51 . 本站查看

    Other cited types(2)

Catalog

    Get Citation
    PDF
    XML
    Article views (3292) PDF downloads (40) Cited by(3)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return