Volume 16 Issue 3
Jun.  2020
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HUANG Zhikang, JIANG Shigui, ZHOU Falin, HUANG Jianhua, YANG Qibin, JIANG Song, LI Yundong, YANG Lishi. Establishment of a sex identification method for black tiger shrimp (Penaeus monodon) in embryonic and early postembryonic development based on an InDel molecular marker[J]. South China Fisheries Science, 2020, 16(3): 113-118. DOI: 10.12131/20190222
Citation: HUANG Zhikang, JIANG Shigui, ZHOU Falin, HUANG Jianhua, YANG Qibin, JIANG Song, LI Yundong, YANG Lishi. Establishment of a sex identification method for black tiger shrimp (Penaeus monodon) in embryonic and early postembryonic development based on an InDel molecular marker[J]. South China Fisheries Science, 2020, 16(3): 113-118. DOI: 10.12131/20190222
shu

Establishment of a sex identification method for black tiger shrimp (Penaeus monodon) in embryonic and early postembryonic development based on an InDel molecular marker

  • 1.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 210306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

  • 3.

    Shenzhen Experimental Station of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

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  • Received Date: November 01, 2019
  • Revised Date: February 10, 2020
  • Accepted Date: February 24, 2020
  • Available Online: April 28, 2020
    Graphical Abstract
    • Abstract

  • In order to distinguish the sex of Penaeus monodon at embryonic and early stages of postembryonic development, we collected the individuals of fertilized eggs, Nauplii larvae, Zoea larvae, Mysis larvae, PL1 (1 day post-larvae), PL30 (30 day post-larvae) and subadults, tracked them by an improved Insertion/Deletion (InDel) marker, then established the detection methods of sequencing and fluorescence quantitative PCR. The results indicate that a sex-specific sequences could be obtained by this method. The result of sex discriminating of subadult shrimps by sequencing method (accuracy rate of 100%) was consistent with that by external observation method. Sex-specific sequences could be amplified from the samples of fertilized eggs, Nauplii larvae, Zoea larvae, Mysis larvae and post-larvae. Furthermore, we explored a rapid discriminating method based on the melting curve of qRT-PCR (Quantitative real-time polymerase chain reaction) with SYBR Green, and the value of melting temperature (Tm) of sex-specific sequences was (79.10±0.10) ℃ for male and (78.45±0.20) ℃ for female. The sex of individuals of PL30, subadults and Nauplii lavae at Stage VI could be distinguished by the specific melting curve accurately, with an accuracy rate over 96.3%.

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