Volume 16 Issue 3
Jun.  2020
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DING Weidong, CAO Liping, CAO Zheming, BING Xuwen. Effects of acute ammonia nitrogen stress on enzyme activities of gills and digest tract in juvenile mandarin fish (Siniperca chuatsi)[J]. South China Fisheries Science, 2020, 16(3): 31-37. DOI: 10.12131/20190188
Citation: DING Weidong, CAO Liping, CAO Zheming, BING Xuwen. Effects of acute ammonia nitrogen stress on enzyme activities of gills and digest tract in juvenile mandarin fish (Siniperca chuatsi)[J]. South China Fisheries Science, 2020, 16(3): 31-37. DOI: 10.12131/20190188
shu

Effects of acute ammonia nitrogen stress on enzyme activities of gills and digest tract in juvenile mandarin fish (Siniperca chuatsi)

  • Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences/Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi 214081, China

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  • Received Date: September 18, 2019
  • Revised Date: December 09, 2019
  • Accepted Date: January 09, 2020
  • Available Online: January 14, 2020
    Graphical Abstract
    • Abstract

  • We investigated the effects of ammonia stress on the enzyme activities in gills and digestive tract of juvenile mandarin fish (Siniperca chuatsi) with body mass of (13.31±0.49) g and body length of  (9.73±0.46) cm to reveal their physiological changes at  temperature of (22.0±0.5) ℃ and ammonia concentration of 48.65 mg·L−1. The results show that the juveniles under acute ammonia nitrogen stress had undergone significant changes in the activity levels of Na+/K+-ATPase, respiratory metabolic enzymes and digestive enzymes. The Na+/K+-ATPase activity was significantly different from that of the control group, which first increased and then decreased with time. The activity of respiratory metabolism enzyme increased under stress. Digestive enzymes in the stomach and digestive tract were significantly affected too. AMS activity first decreased and then increased, while pepsin and LPS first  increased  and then decreased. The results suggest that ammonia nitrogen stress can cause a variety of impairments in fish body, including deterioration of antioxidant system, physiological metabolism, gill tissue, respiratory function and detoxification function. In order to keep impairment less, the digestive and anaerobic metabolism enzyme activities should be activated to higher level to produce energy for the body's resistance to stress response.

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