Volume 15 Issue 3
Jun.  2019
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XIA Jingjin, CHEN Jianwu, SONG Yi, LIU Yongtao, DONG Jing, WU Yunan, AI Xiaohui. Identification of antibacterial substances from Bacillus amyloliquefaciens HE and analysis of antibacterial characteristics[J]. South China Fisheries Science, 2019, 15(3): 41-49. DOI: 10.12131/20190054
Citation: XIA Jingjin, CHEN Jianwu, SONG Yi, LIU Yongtao, DONG Jing, WU Yunan, AI Xiaohui. Identification of antibacterial substances from Bacillus amyloliquefaciens HE and analysis of antibacterial characteristics[J]. South China Fisheries Science, 2019, 15(3): 41-49. DOI: 10.12131/20190054
shu

Identification of antibacterial substances from Bacillus amyloliquefaciens HE and analysis of antibacterial characteristics

  • 1.

    College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

  • 2.

    Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China

  • 3.

    Key Laboratory of Quality and Safety Control for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, China

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  • Received Date: March 11, 2019
  • Revised Date: March 26, 2019
  • Accepted Date: April 07, 2019
  • Available Online: April 18, 2019
    Graphical Abstract
    • Abstract

  • To investigate the antibacterial substances from Bacillus amyloliquefaciens HE and antibacterial characteristics, we synthetized the genes related to lipopeptides synthesis by PCR detection according to the stability of B. amyloliquefaciens fermentation broth, and further analyzed the active substances by UPLC-Q-TOF-MS. Then we applied the inhibition zone method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lipopeptide against Aeromonas hydrophila, whose antimicrobial activity was discussed from the microscopic structure. The antimicrobial active substances were stable against high temperature, acid, acid-base and protease. The PCR with four primer pairs were cloned and sequenced. The BLAST analysis shows that the ituA, fenB, sfP and mycB genes existed in the genome of B. amyloliquefaciens HE. Three kinds of lipopetides (Surfactin, Iturin and Fengycin) were identified in the antimicrobial extract by mass spectrum analysis. The MIC and MBC of antimicrobial lipopetides against A. hydrophila were both 137.97 μg·mL−1 by inhibition zone method. The microscopic characteristics indicate that lipopeptides can cause membrane collapse and holes in A. hydrophila cells. The results provide references for the structural identification and safety evaluation of active metabolites of B. amyloliquefaciens.

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    • References (33)
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