Volume 15 Issue 4
Aug.  2019
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SUN Yongxu, DONG Hongbiao, WANG Wenhao, CAO Ming, DUAN Yafei, LI Hua, LIU Qingsong, ZHANG Jiasong. Effects of periodic hypoxia stress on intestinal microflora structure of Lateolabrax maculatus[J]. South China Fisheries Science, 2019, 15(4): 46-52. DOI: 10.12131/20190021
Citation: SUN Yongxu, DONG Hongbiao, WANG Wenhao, CAO Ming, DUAN Yafei, LI Hua, LIU Qingsong, ZHANG Jiasong. Effects of periodic hypoxia stress on intestinal microflora structure of Lateolabrax maculatus[J]. South China Fisheries Science, 2019, 15(4): 46-52. DOI: 10.12131/20190021
shu

Effects of periodic hypoxia stress on intestinal microflora structure of Lateolabrax maculatus

  • 1.

    Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Guangdong Provincial Fishery Germplasm Conservation Center, Guangzhou 511453, China

  • 4.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

More Information
  • Received Date: December 20, 2018
  • Revised Date: January 28, 2019
  • Accepted Date: April 09, 2019
  • Available Online: April 18, 2019
    Graphical Abstract
    • Abstract

  • Based on the technology of Illumina MiSeq high throughput sequencing, we analyzed the effects of periodic hypoxia stress on intestinal microflora structure of Lateolabrax maculatus, so as to provide references for the study of the adaptation mechanism of intestinal flora of the juveniles to environmental hypoxia. The results show that hypoxia caused a significant increase in the diversity and richness of intestinal microflora (P<0.05). The species richness was rather different between the two groups. The operational taxonomic units (OTUs) of hypoxic group were significantly more than those of normoxic group (P<0.05). At the level of Class classification, the dominant bacteria of both groups were Proteobacteria, Firmicutes and Bacteroidetes. Compared with the normoxic group, the relative abundance of Proteobacteria decreased significantly in hypoxic group, while that of Bacteroidetes increased significantly (P<0.05). At the level of Genus classification, the relative abundance of Alphaproteobacteria and Bacilli in hypoxic group decreased significantly, while those of Clostridia, Gammaproteobacteria and Bacteroidia increased significantly (P<0.05). Periodic hypoxia stress also led to an increase of relative abundance of anaerobes or facultative anaerobes (Anaerolineaceae, Lachnospiraceae and Ruminococcaceae) and oxygen-producing bacteria (Chlorobiaceae) in the intestine of L. maculatus.

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