LI Chao, KE Changliang, GU Xiaoli, LI Huiqing, LI Zhiguang, HUANG Ke, LI Liudong, LI Laihao. Preliminary study of antibacterial effects of fish anesthetic eugenol on Vibrio anguillarum[J]. South China Fisheries Science, 2019, 15(2): 58-63. DOI: 10.12131/20180194
Citation: LI Chao, KE Changliang, GU Xiaoli, LI Huiqing, LI Zhiguang, HUANG Ke, LI Liudong, LI Laihao. Preliminary study of antibacterial effects of fish anesthetic eugenol on Vibrio anguillarum[J]. South China Fisheries Science, 2019, 15(2): 58-63. DOI: 10.12131/20180194

Preliminary study of antibacterial effects of fish anesthetic eugenol on Vibrio anguillarum

More Information
  • Received Date: September 03, 2018
  • Revised Date: November 05, 2018
  • Accepted Date: December 16, 2018
  • Available Online: December 19, 2018
  • Fish anesthetic eugenol, which has good antibacterial and bactericidal effects, has presented great efficacy on the food borne pathogens as well as the pathogenic bacteria in aquaculture. In order to investigate the antibacterial effect of eugenol on the typical pathogenic bacteria in aquaculture, we studied the antibacterial effect of eugenol on Vibrio anguillarum. After 24-hour incubation, the diameter of the inhibition zone was (21.13±0.74) mm at 6 400 μg·mL–1 of eugenol, indicating that V. anguillarum was extremely sensitive to eugenol. It is shown that V. anguillarum did not grow while the eugenol concentration was over 400 μg·mL–1. Hence, the minimum inhibitory concentration (MIC) was 400 μg·mL–1 of eugenol against the bacteria. The solution in the cells of 400 μg·mL–1, 800 μg·mL–1 and 1 600 μg·mL–1 were continually incubated for 24 h after being scribbled on the plate surface. When the concentration of eugenol were over 800 μg·mL–1, no V. anguillarum grew in the plates. It is indicated that the minimum bactericidal concentration (MBC) of eugenol against V. anguillarum was 800 μg·mL–1, and eugenol has excellent antibacterial effects on V. anguillarum.

  • [1]
    王凤青, 孙玉增, 任利华, 等. 海水养殖中水产动物主要致病弧菌研究进展[J]. 中国渔业质量与标准, 2018, 8(2): 49-56. doi: 10.3969/j.issn.2095-1833.2018.02.007
    [2]
    尹清干. 鳗弧菌(Vibrio anguillarum)生物膜的形成及三种中草药对其干预的研究[D]. 上海: 上海海洋大学, 2017: 2-5.
    [3]
    靖凯霖, 徐永平, 李媛, 等. 卵黄抗体在水产养殖病害防治及水产品保鲜中的应用研究进展[J]. 水产科学, 2016, 35(2): 185-190.
    [4]
    XU Z N, WANG Y, HAN Y, et al. Mutation of a novel virulence-related gene mltD in Vibrio anguillarum, enhances lethality in zebra fish[J]. Res Microbiol, 2011, 162(2): 144-150. doi: 10.1016/j.resmic.2010.08.003
    [5]
    TORANZO A E, BARJA J L. A review of the taxonomy and seroepizootiology of Vibrio anguillarum, with special reference to aquaculture in the northwest of Spain[J]. Dis Aquat Organ, 1990, 9(1): 73-82.
    [6]
    PLAZA N, CASTILLO D, PÉREZ-REYTOR D A, et al. Bacteriophages in the control of pathogenic vibrios[J]. Electron J Biotechn, 2018, 31: 24-33. doi: 10.1016/j.ejbt.2017.10.012
    [7]
    陆志科, 谢碧霞. 植物源天然食品防腐剂的研究进展[J]. 食品工业科技, 2003(1): 94-96, 93. doi: 10.3969/j.issn.1002-0306.2003.01.041
    [8]
    曾荣, 陈金印, 林丽超. 丁香精油及丁香酚对食品腐败菌的抑菌活性研究[J]. 江西农业大学学报, 2013(4): 852-857. doi: 10.3969/j.issn.1000-2286.2013.04.033
    [9]
    BURT S. Essential oils: their antibacterial properties and potential applications in foods: a review[J]. Int J Food Microbiol, 2004, 94(3): 223-253. doi: 10.1016/j.ijfoodmicro.2004.03.022
    [10]
    JALALI N, ARIIAI P, FATTAHI E. Effect of alginate/carboxyl methyl cellulose composite coating incorporated with clove essential oil on the quality of silver carp fillet and Escherichia coli O157: H7 inhibition during refrigerated storage[J]. J Food Sci Tech, 2016, 53(1): 757-765. doi: 10.1007/s13197-015-2060-4
    [11]
    刁文睿. 公丁香油脂的体外抗氧化、抑菌活性及抑菌机理研究[D]. 临汾: 山西师范大学, 2015: 25-26.
    [12]
    GUTIERREZ J, RODRIGUEZ G, BARRY-RYAN C A. Efficacy of plant essential oils against foodborne pathogens and spoilage bacteria associated with ready-to-eat vegetables: antimicrobial and sensory screening[J]. J Food Prot, 2008, 71(9): 1846-1854. doi: 10.4315/0362-028X-71.9.1846
    [13]
    ALBERTOS I, RICO D, MARÍA DIEZ A, et al. Effect of edible chitosan/clove oil films and high-pressure processing on the microbiological shelf life of trout fillets[J]. J Sci Food Agric, 2015, 95(14): 2858-2865. doi: 10.1002/jsfa.2015.95.issue-14
    [14]
    VRINDA MENON K, GARG S R. Inhibitory effect of clove oil on Listeria monocytogenes in meat and cheese[J]. Food Microbiol, 2001, 18(6): 647-650. doi: 10.1006/fmic.2001.0430
    [15]
    BOIJINK C D, QUEIROZ C A, CHAGAS E C, et al. Anesthetic and anthelminthic effects of clove basil (Ocimum gratissimum) essential oil for tambaqui (Colossoma macropomum)[J]. Aquaculture, 2016, 457: 24-28. doi: 10.1016/j.aquaculture.2016.02.010
    [16]
    方晓磊, 柯常亮, 李刘冬, 等. 丁香酚辅助鲜活草鱼处理和运输的剂量研究[J]. 食品工业科技, 2017, 38(17): 275-278.
    [17]
    SUTILI F J, KREUTZ L C, NORO M A, et al. The use of eugenol against Aeromonas hydrophila and its effect on hematological and immunological parameters in silver catfish (Rhamdia quelen)[J]. Vet Immunol Immunopathol, 2014, 157(3/4): 142-148.
    [18]
    BANDEIRA J, PÊS T S, SACCOL E M, et al. Potential uses of Ocimum gratissimum and Hesperozygis ringens essential oils in aquaculture[J]. Ind Crops Prod, 2017, 97: 484-491. doi: 10.1016/j.indcrop.2016.12.040
    [19]
    ROMERO J, FEIJOÓ C G, NAVARRETE P. Antibiotics in aquaculture-use, abuse and alternatives[M]//Health and environment in aquaculture. Slavka Krautzeka: Publisher InTech, 2012: 165-168.
    [20]
    张永刚, 栾林林, 任海, 等. 中草药对大菱鲆源哈维氏弧菌的体外抑菌效果[J]. 淡水渔业, 2018, 48(3): 78-82. doi: 10.3969/j.issn.1000-6907.2018.03.013
    [21]
    沈建忠, 于康震. 食品中抗菌药物残留的化学分析[M]. 北京: 中国农业出版社, 2017: 5-6.
    [22]
    陈丽婷, 郇志利, 王晓清, 等. 中草药添加剂在水产养殖中的应用研究进展[J]. 水产科学, 2014, 33(3): 190-194. doi: 10.3969/j.issn.1003-1111.2014.03.012
    [23]
    崔惠敬, 孟玉霞, 赵前程, 等. 植物精油在鱼类养殖中的研究与应用[J]. 水产科学, 2018, 37(4): 564-570.
    [24]
    SANTOS L, RAMOS F. Antimicrobial resistance in aquaculture: current knowledge and alternatives to tackle the problem[J]. Int J Antimicrob Agents, 2018, 52(2): 135-143. doi: 10.1016/j.ijantimicag.2018.03.010
    [25]
    王玉堂. 禁用渔药化合物及其危害[J]. 中国水产, 2017(1): 76-80.
    [26]
    ELMAHDI S, DASILVA L V, PARVEEN S. Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: a review[J]. Food Microbiol, 2016, 57: 128-134. doi: 10.1016/j.fm.2016.02.008
    [27]
    童国忠, 石亚素, 马剑茵, 等. 中草药对海水养殖黄鱼病原弧菌抑菌效果研究[J]. 水产科学, 2007, 26(8): 436-439. doi: 10.3969/j.issn.1003-1111.2007.08.003
    [28]
    刘克奉, 刘克明, 刘鑫. 不同中草药对两株致病菌的抑菌作用研究[C]//2012全国海水养殖学术研讨会论文集. 北京: 海洋出版社, 2012: 302-316.
    [29]
    DIREKBUSARAKOM S. Application of medicinal herbs to aquaculture in Asia[J]. Walailak J Sci Tech, 2004, 1(1): 7-13.
    [30]
    马艳玲, 李海贤, 曾荣. 丁香酚对金黄色葡菌球菌抗菌作用的探究[J]. 中国酿造, 2017(8): 130-133.
    [31]
    QIU J Z, FENG H H, LU J, et al. Eugenol reduces the expression of virulence-related exoproteins in Staphylococcus aureus[J]. Appl Environ Microbiol, 2010, 76(17): 5846-5851. doi: 10.1128/AEM.00704-10
    [32]
    唐小辉. 肉桂醛、丁香酚及其结构类似物的抑菌活性与化学结构的关系[D]. 湘潭: 湘潭大学, 2013: 38-50.
    [33]
    FILGUEIRAS C T, VANETTI M D. Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes[J]. Braz Arch Biol Techn, 2006, 49(3): 405-409. doi: 10.1590/S1516-89132006000400008
    [34]
    田琦. 丁香酚和茶多酚对空肠弯曲菌抑菌机理研究[D]. 天津: 天津科技大学, 2014: 39.
    [35]
    RATTANACHAIKUNSOPON P, PHUMKHACHORN P. Protective effect of clove oil-supplemented fish diets on experimental Lactococcus garvieae infection in tilapia[J]. Biosci Biotechnol Biochem, 2009, 73(9): 2085-2089.
    [36]
    ZHANG Y, WANG Y, ZHU X J, et al. Antibacterial and antibiofilm activities of eugenol from essential oil of Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf against periodontal pathogen Porphyromonas gingivitis[J]. Microb Pathogenesis, 2017, 113: 396-402. doi: 10.1016/j.micpath.2017.10.054
    [37]
    PRAMOD K, ANSARI S H, ALI J. Eugenol: a natural compound with versatile pharmacological actions[J]. Nat Prod Commun, 2010, 5(12): 1999-2006.
    [38]
    苏明明, 孙兴权, 杨春光, 等. 渔用麻醉剂MS-222、丁香酚在鲜活水产品运输中的应用及检测方法研究进展[J]. 食品安全质量检测学报, 2015(1): 25-29.
    [39]
    方晓磊, 柯常亮, 刘奇, 等. 水产品中丁香酚残留的人体健康风险分析[J]. 水产科学, 2018, 37(1): 140-144.
  • Related Articles

    [1]LIU Yuhang, XIANG Huan, HUANG Hui, WEI Ya, CEN Jianwei, PAN Chuang, LI Chunsheng, ZHAO Yongqiang, HAO Shuxian. Optimization of process and mechanism of extracting astaxanthin from shrimp shells with deep eutectic solvent[J]. South China Fisheries Science, 2024, 20(3): 164-172. DOI: 10.12131/20230225
    [2]WANG Jiamei, TANG Zhenzhao, DING Ling, JIA Xiaoping, ZHANG Zhongzhe. Study on local scour of artificial reef based on flume test[J]. South China Fisheries Science, 2020, 16(6): 32-38. DOI: 10.12131/20200074
    [3]GU Liangbin, XU Liwen, FENG Juan, SU YouLu, LIU Guangfeng, GUO Zhixun. Identification and drug sensitive test of bacterial Pathogens from Plectropomus leopardus with tail fester disease[J]. South China Fisheries Science, 2015, 11(4): 71-80. DOI: 10.3969/j.issn.2095-0780.2015.04.011
    [4]LIU Jian, HUANG Hongliang, WU Yue, RAO Xin, LI Lingzhi, CHEN Shuai, YANG Jialiang, ZHOU Bin. Model test of hydrodynamic characteristics of two types of vertical cambered slotted otter boards[J]. South China Fisheries Science, 2015, 11(1): 68-74. DOI: 10.3969/j.issn.2095-0780.2015.01.010
    [5]YE Le, ZHAO Wang, WU Kaichang. Acute toxicity tests of three antiparasitic drugs to juvenile snail of Babylonia areolata[J]. South China Fisheries Science, 2014, 10(4): 22-26. DOI: 10.3969/j.issn.2095-0780.2014.04.004
    [6]DING Aixia, HE Yi′er. Test on release and proliferation of Pseudosciaena crocea in Daiquyang sea area[J]. South China Fisheries Science, 2011, 7(1): 73-77. DOI: 10.3969/j.issn.2095-0780.2011.01.012
    [7]YANG Xueming, ZHANG Li, LI Youning, CHEN Mingqiang, GAN Xi. Primary test on artificial spawning induction and larvae rearing of Holothuria scabra[J]. South China Fisheries Science, 2011, 7(1): 39-44. DOI: 10.3969/j.issn.2095-0780.2011.01.006
    [8]HU Zehui, XU Junzhuo, WANG Yuebin, CHAI Xuejun. Optimization of SRAP-PCR system for Portunus trituberculatus based on orthogonal experimental design[J]. South China Fisheries Science, 2010, 6(5): 68-73. DOI: 10.3969/j.issn.1673-2227.2010.05.011
    [9]YANG Qibin, LI Xiaolan, SUN Miaomiao, WEN Weigeng, HUANG Jianhua. Methods of testing vitality of postlarvae of Penaeus monodon[J]. South China Fisheries Science, 2010, 6(1): 12-18. DOI: 10.3969/j.issn.1673-2227.2010.01.003
    [10]XU Jing. Study on the extraction of natural astaxanthin by orthogonal test[J]. South China Fisheries Science, 2008, 4(2): 69-74.

Catalog

    Article views PDF downloads Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return