Volume 13 Issue 1
Apr.  2017
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WANG Zhenzhen, HUANG Guiju, FAN Sigang, LIU Bosuo, ZHANG Bo, SU Jiaqi, YU Dahui. Molecular cloning and expression profiles of matrilin-1 in pearl oyster (Pinctada fucata)[J]. South China Fisheries Science, 2017, 13(1): 76-84. DOI: 10.3969/j.issn.2095-0780.2017.01.010
Citation: WANG Zhenzhen, HUANG Guiju, FAN Sigang, LIU Bosuo, ZHANG Bo, SU Jiaqi, YU Dahui. Molecular cloning and expression profiles of matrilin-1 in pearl oyster (Pinctada fucata)[J]. South China Fisheries Science, 2017, 13(1): 76-84. DOI: 10.3969/j.issn.2095-0780.2017.01.010
shu

Molecular cloning and expression profiles of matrilin-1 in pearl oyster (Pinctada fucata)

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: March 24, 2016
  • Revised Date: May 05, 2016
  • Accepted Date: July 03, 2016
    Graphical Abstract
    • Abstract

  • Matrilin-1 gene was cloned from the Pinctada fucata based on its transcriptome sequences and named as Pfmatrilin-1. The whole length of Pfmatrilin-1 was 2 036 bp, including a 1 194 bp ORF encoding 397 amino acids, in which there were a signal peptide and two VWA (von Willebrand factor A) motifs. Phylogeny analysis proves that the matrilins in vertebrates and invertebrates were of distant relationship. The qRT-PCR tissue expression analysis indicates that Pfmatrilin-1 was expressed in all the tested tissues with significantly high expression level in blood cells (P < 0.05).The Pf-matrilin-1 was also continuously expressed from trochophore to metamorphosis stages, reaching the peak at pediveliger stage (P < 0.05).The prodissoconchⅡformed during pediveliger stage, so the Pfmatrilin-1 may be involved in regulating the formation of prodissoconchⅡ. Thus, it is proved that the Pfmatrilin-1 plays an important role of biomineralization in P.fucata.

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    • References (33)
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