Volume 12 Issue 1
Feb.  2016
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WEI Jihai, ZHAO Jinliang, WU Junwei, LUO Mingkun, YE Wei, FU Yun, CHEN Huichong. Genetic characterization of Oreochromis niloticus (♀) × Sarotherodon melanotheron (♂) hybrid F2 and F3 by microsatellite analysis[J]. South China Fisheries Science, 2016, 12(1): 30-35. DOI: 10.3969/j.issn.2095-0780.2016.01.005
Citation: WEI Jihai, ZHAO Jinliang, WU Junwei, LUO Mingkun, YE Wei, FU Yun, CHEN Huichong. Genetic characterization of Oreochromis niloticus (♀) × Sarotherodon melanotheron (♂) hybrid F2 and F3 by microsatellite analysis[J]. South China Fisheries Science, 2016, 12(1): 30-35. DOI: 10.3969/j.issn.2095-0780.2016.01.005
shu

Genetic characterization of Oreochromis niloticus (♀) × Sarotherodon melanotheron (♂) hybrid F2 and F3 by microsatellite analysis

  • 1.

    Lab.of Freshwater Fisheries Germplasm Resources, Ministry of Agriculture; Shanghai Ocean University, Shanghai 201306, China

  • 2.

    Guangdong Freshwater Famous Fish Seed Breeding Center, Guangzhou 511453, China

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  • Received Date: January 28, 2015
  • Revised Date: April 15, 2015
    Graphical Abstract
    • Abstract

  • A preliminary comparative analysis of genetic characteristics between F2 and F3 hybrid populations of Oreochromis niloticus♀×Sarotherodon melanotheron♂ was conducted by using microsatellite markers from the second generation genetic linkage map of Nile tilapia. The results show that 22 pairs of primers were amplified and no polyploidy individual was detected in F2 and F3 populations, seven different amplified loci were screened out in hybrids F2 and F3 populations from 28 loci. In F2 hybrid population, average allele was 2.71;average polymorphic information content was 0.466;observed heterozygosity was 0.632. In F3 hybrids, the average allele was 2.14;average polymorphic information content was 0.370;observed heterozygosity was 0.432. The genetic heterozygosity of F3 population was lower than that of F2 population. In F3 population, the allele number at four loci was identical with F2 population, while the allele numbers at three loci were less than that of F2 population. Moreover, there were two homozygous loci in F3 population; the change of alleles in F3 population showed a homozygous trend. The results provide some fundamental data for inheritance of different generations and tilapia hybridization utilization.

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    • References (21)
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