Volume 10 Issue 2
Apr.  2014
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XIAO Yayuan, XU Shannan, LIU Yong, LI Chunhou. Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)[J]. South China Fisheries Science, 2014, 10(2): 61-66. DOI: 10.3969/j.issn.2095-0780.2014.02.009
Citation: XIAO Yayuan, XU Shannan, LIU Yong, LI Chunhou. Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)[J]. South China Fisheries Science, 2014, 10(2): 61-66. DOI: 10.3969/j.issn.2095-0780.2014.02.009
shu

Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)

  • Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; Key Lab. of Fishery Ecology Environment, Guangdong Province; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: October 20, 2013
  • Revised Date: November 24, 2013
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    • Abstract

  • Green mussels (Perna viridis), exposed to 0 μg · L-1, 5 μg · L-1, 10 μg · L-1, 50 μg · L-1 and 100 μg · L-1 No.0 diesel oil water-soluble fraction (WSF) for 15 d under semi-hydrostatic simulation, were transferred to clean sea water for 7 d to analyze the gene expression levels of CYP4 by the method of 2-△△Ct combining with real-time quantitative PCR. Results show that WSF obviously induced CYP4 up-regulation and was differentially expressed in both visceral mass and mantle in P.viridis. The expression level of CYP4 was obviously higher in visceral mass than in mantle. The relative gene expression level initially increased then decreased and increased again as time elapsed in those two kinds of tissues. The most significant difference was observed in 50 μg · L-1concentration group (P < 0.01)compared to the control. At the last 7 d, the expression level in visceral mass and mantle decreased quickly and that in some groups gradually returned to normal level by being transferred to clean sea water.

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  • [1]
    MANSUY D. The great diversity of reactions catalyzed by cytochromes P450[J]. Comp Biochem Physiol, 1998, 121(1): 5-14. doi: 10.1016/S0742-8413(98)10026-9
    [2]
    WERCK-REICHHART D, FEYEREISEN R. Cytochromes P450: a success story[J]. Genome Biol, 2000, 1(6): 3003-3004. doi: 10.1186/gb-2000-1-6-reviews3003
    [3]
    许华夏, 李培军, 刘宛. 生物细胞色素P450的研究进展[J]. 农业环境保护, 2002, 21(2): 188-191. doi: 10.3321/j.issn:1672-2043.2002.02.029
    [4]
    BAUSTISTA M A M, TANAKA T. Identification of permethrin-inducible cytochrome P450s from the diamondback moth, Plutella xylostella (L) and the possibility of involvement in permethrin resistance[J]. Pestic Biochem Physiol, 2007, 8(1): 85-93. doi: 10.1016/j.pestbp.2006.06.004
    [5]
    HOARAU P, DAMIENS G, ROMEO M, et al. Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution[J]. Comp Biochem Physiol, 2006, 143(2): 196-203. doi: 10.1016/j.cbpc.2006.02.007
    [6]
    石玉荣, 耿建, 王惠, 等. 苯并[a]芘对宫颈癌HeLa细胞细胞色素P4501A1诱导作用[J]. 上海交通大学学报: 医学版, 2010, 30(12): 1513-1516. doi: 10.3969/j.issn.1674-8115.2010.12.016
    [7]
    CAPDEVILA J H, FALCK J R. The CYP 450 arachidonic acid monooxygenases: from cell signaling to blood pressure regulation[J]. Biochem Biophys Res Commun, 2001, 285(3): 571-576. doi: 10.1006/bbrc.2001.5167
    [8]
    DAVID J P, BOYER S, MESNEAU A, et al. Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics[J]. Insect Biochem Mol Biol, 2006, 36(5): 410-420. doi: 10.1016/j.ibmb.2006.02.004
    [9]
    国家海洋局. 海洋监测规范(GB 17378.1-2007)[M]北京: 海洋出版社, 2007: 153-156. https://xueshu.baidu.com/usercenter/paper/show?paperid=1f0d0690t23p08w0w94s0j80dm465256&site=xueshu_se&hitarticle=1
    [10]
    周驰, 李纯厚, 张为民, 等. 翡翠贻贝CYP4基因克隆及其表达水平分析[J]. 热带海洋学报, 2010, 29(4): 82-88. doi: 10.3969/j.issn.1009-5470.2010.04.013
    [11]
    LICAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real time quantitative PCR and the 2-△△Ct method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
    [12]
    龙敏明, 黄桂菊, 邹记兴, 等. 育珠对合浦珠母贝N19和Prismalin-14基因表达水平的影响[J]. 南方水产科学, 2013, 9(5): 58-63. doi: 10.3969/j.issn.2095-0780.2013.05.010
    [13]
    吴益东, 沈文飚. 棉铃虫六龄幼虫微粒体细胞色素P450和细胞色素b5的测定及组织分布[J]. 农业生物技术学报, 1997, 5(3): 297-301. https://xueshu.baidu.com/usercenter/paper/show?paperid=715b6198a4baf17dd0252ac6f8d7ad4b&site=xueshu_se
    [14]
    陈立兵, 葛卫红, 林洁. 细胞色素P450 mRNA表达研究进展[J]. 医学研究杂志, 2007, 36(7): 79. doi: 10.3969/j.issn.1673-548X.2007.07.004
    [15]
    徐增光, 文冠华, 高雅娟. 苯并(a)芘对小鼠肺组织细胞色素P450 1A表达的影响[J]. 华中科技大学学报: 医学版, 2005, 34(4): 404-406. doi: 10.3870/j.issn.1672-0741.2005.04.007
    [16]
    FISHER T, CKANE M. Induction of cytochrome P450 activity in individual Chironomus riparius Meigen larvae exposed to xenobiotics[J]. Ecotoxicol Environ Saf, 2003, 54(1): 1-6. doi: 10.1016/S0147-6513(02)00031-3
    [17]
    BURNETT K G, BAIN L J. Fundus as the premier teleost model in environmental biology: opportunities for new insights using genomics[J]. Comp Biochem Physiol, 2007, 2(4): 257-286. doi: 10.1016/j.cbd.2007.09.001
    [18]
    周驰, 李纯厚, 张为民. 翡翠贻贝肾CYP4基因表达受多氯联苯影响的研究[J]. 安徽农业科学, 2008, 36(22): 9418-9422. doi: 10.3969/j.issn.0517-6611.2008.22.036
    [19]
    STEGEMAN J J, SMOLOWITZ R M, HAHN M E. Immunohistochemical localization of environmentally induced cytochrome P450IAl in multiple organs of the marine teleost Stenotomus chrysops(scup)[J]. Toxicol Appl Pharmacol, 1991, 110(3): 486-504. doi: 10.1016/0041-008X(91)90049-K
    [20]
    张薇, 宋玉芳, 孙铁珩. 菲和芘对蚯蚓(Eisenia fetida)细胞色素P450和抗氧化酶系的影响[J]. 环境化学, 2007, 26(2): 202-206. doi: 10.3321/j.issn:1672-2043.2009.02.021
    [21]
    王磊, 宋玉芳, 张薇, 等. 蚯蚓(Eisenia fetida)细胞色素P450及抗氧化酶系对环境浓度苯并(a)芘的响应[J]. 农业环境科学学报, 2009, 28(2): 337-342. doi: 10.3321/j.issn:1672-2043.2009.02.021
    [22]
    肖雅元, 李纯厚, 徐姗楠, 等. 水溶性石油烃组分对翡翠贻贝的毒性效应研究[J]. 农业环境科学学报, 2012, 31(3): 598-606.
    [23]
    王燕红, 王东, 李兵, 等. 野桑蚕细胞色素P450家族CYP4M5基因的克隆和诱导表达[J]. 蚕业科学, 2009, 35(1): 78-83. doi: 10.3969/j.issn.0257-4799.2009.01.013
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