Volume 8 Issue 6
Dec.  2012
Turn off MathJax
Article Contents
Abstract
References
ZHANG Yan, WU Yanyan, LI Laihao, YANG Xianqing, SHAO Zhengyi. Identification of a symbiotic and epiphyte lactic acid bacterium strain from Pinctada fucata by 16S rDNA sequencing[J]. South China Fisheries Science, 2012, 8(6): 9-15. DOI: 10.3969/j.issn.2095-0780.2012.06.002
Citation: ZHANG Yan, WU Yanyan, LI Laihao, YANG Xianqing, SHAO Zhengyi. Identification of a symbiotic and epiphyte lactic acid bacterium strain from Pinctada fucata by 16S rDNA sequencing[J]. South China Fisheries Science, 2012, 8(6): 9-15. DOI: 10.3969/j.issn.2095-0780.2012.06.002
shu

Identification of a symbiotic and epiphyte lactic acid bacterium strain from Pinctada fucata by 16S rDNA sequencing

  • 1.

    National R&D Center for Aquatic Product Processing; Key Lab. of Aquatic Product Processing, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Shnghai Ocean University, Shanghai 201306, China

More Information
  • Received Date: May 09, 2012
  • Revised Date: July 15, 2012
    Graphical Abstract
    • Abstract

  • The strain F9, which can produce antibacterial substances, was isolated from the Pinctada fucata mucus and finally identified as Lactobacillus coryniformis by 16S rDNA sequence analysis. The antimicrobial spectrum of strain F9 was investigated with agar diffusion method. The results show that the antimicrobial activity of the supernatant is not due to the existence of hydrogen peroxide and organic acid in the solution, and can significantly inhibit various common foodborne pathogens such as Staphyloccocus aureus. It is inferred that the antibacterial substances are proteins with molecular weight of 5~10 kDa based on ultrafiltration analysis and protease hydrolysis.

    • FullText(HTML)
    • References (20)
  • [1]
    宫晓静, 吴燕燕. 海洋无脊椎动物抗菌肽研究进展及其在食品保鲜中的应用[J]. 生物技术通报, 2011, 224(3): 27-32. https://wenku.baidu.com/view/d849be1e6bd97f192279e943?fr=xueshu_top&_wkts_=1732080737617&needWelcomeRecommand=1

    GONG Xiaojing, WU Yanyan. Review on research progresses and application in the food preservation of antimicrobial peptides from marine invertebrates[J]. Biotechnol Bull, 2011, 224(3): 27-32. (in Chinese) https://wenku.baidu.com/view/d849be1e6bd97f192279e943?fr=xueshu_top&_wkts_=1732080737617&needWelcomeRecommand=1
    [2]
    汤健, 刘文广, 林坚士, 等. 9个马氏珠母贝家系的中期生长性状评估[J]. 南方水产科学, 2011, 7(5): 30-36. doi: 10.3969/j.issn.2095-0780.2011.05.005

    TANG Jian, LIU Wenguang, LIN Jianshi, et al. Evaluation on mid-term growth of 9 families of pearl oyster Pinctada facata[J]. South China Fish Sci, 2011, 7(5): 30-36. (in Chinese) doi: 10.3969/j.issn.2095-0780.2011.05.005
    [3]
    张岩, 吴燕燕, 李来好, 等. 酶法制备海洋活性肽及其功能活性研究进展[J]. 生物技术通报, 2012, 225(3): 42-48. https://www.cnki.com.cn/Article/CJFDTotal-swjt201203006.htm

    ZHANG Yan, WU Yanyan, LI Laihao, et al. Review on research progresses in bioactive peptides from marine protein by enzyme[J]. Biotechnol Bull, 2012, 225(3): 42-48. (in Chinese) https://www.cnki.com.cn/Article/CJFDTotal-swjt201203006.htm
    [4]
    吴燕燕, 宫晓静, 李来好, 等. 风味蛋白酶水解合浦珠母贝肉制备抗菌肽人工神经网络法优化工艺[J]. 食品科学, 2011, 32(20): 63-68. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1zcGt4MjAxMTIwMDE0GghkY3UyeTE1aw%3D%3D

    WU Yanyan, GONG Xiaojing, LI Laihao, et al. Process optimization for preparation of antimicrobial peptides from Pinctada fucata muscle by flavourzyme hydrolysis using artificial production neural network[J]. Food Sci, 2011, 32(20): 63-68. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1zcGt4MjAxMTIwMDE0GghkY3UyeTE1aw%3D%3D
    [5]
    杜荣茂. 天然食品防腐剂[J]. 中国食品添加剂, 2003(2): 68-74. doi: 10.3969/j.issn.1006-2513.2003.02.018

    DU Rongmao. Natural food preservatives[J]. China Food Addit, 2003(2): 68-74. (in Chinese) doi: 10.3969/j.issn.1006-2513.2003.02.018
    [6]
    GÁLVEZ A, ABRIOUEL H, LÓPEZ R L, et al. Bacteriocin-based strategies for food biopreservation[J]. Int J Food Microbiol, 2007, 120(1/2): 51-70. doi: 10.1016/j.ijfoodmicro.2007.06.001
    [7]
    赵婷, 李辉, 程池. 16S rRNA二级结构分析在微生物分类鉴定中的应用[J]. 食品与发酵工业, 2011, 37(12): 105-108. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5zcHlmeDIwMTExMjAzMhoIYW1lbGdsZG8%3D

    ZHAO Ting, LI Hui, CHENG Chi. 16S rRNA secondary structure analysis in microbial classification and identification of application[J]. Food Ferment Ind, 2011, 37(12): 105-108. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg5zcHlmeDIwMTExMjAzMhoIYW1lbGdsZG8%3D
    [8]
    东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 289-292.

    DONG Xiuzhu, CAI Miaoying. Common bacteria system identification manual[M]. Beijing: Science Press, 2001: 289-292. (in Chinese)
    [9]
    WANG Qiong, GARRITY G M, TIEDJE J M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Appl Environ Microbiol, 2007, 73(16): 5261-5267. doi: 10.1128/AEM.00062-07
    [10]
    王江勇, 孙秀秀, 王瑞旋, 等. 杂色鲍肌肉萎缩症病原菌的分离鉴定及系统发育分析[J]. 南方水产, 2010, 6(5): 21-26. doi: 10.3969/j.issn.1673-2227.2010.05.004

    WANG Jiangyong, SUN Xiuxiu, WANG Ruixuan, et al. Isolation, identification and phylogenetic analysis of pathogen from Haliotis diversicolor Reeve with withering syndrome[J]. South China Fish Sci, 2010, 6(5): 21-26. (in Chinese) doi: 10.3969/j.issn.1673-2227.2010.05.004
    [11]
    李琳, 贾士儒, 谭之磊, 等. 嗜热链球菌CGMCC 1.1864所产的一种新型细菌素ST9[J]. 微生物学通报, 2010, 37(3): 349-354. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg93c3d4dGIyMDEwMDMwMDUaCGdzMXBod2c4

    LI Lin, JIA Shiru, TAN Zhilei, et al. Thermophilin ST9, a new bacteriocin produced by Streptococcus thermophilus CGMCC 1.1864[J]. Microbiol China, 2010, 37(3): 349-354. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg93c3d4dGIyMDEwMDMwMDUaCGdzMXBod2c4
    [12]
    吴燕燕, 尚军, 李来好, 等. 合浦珠母贝肉短肽的分离及其抗氧化活性研究[J]. 食品工业科技, 2012, 32(7): 123-126. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg9zcGd5a2oyMDEyMDcwMzQaCHdidHlyODh3

    WU Yanyan, SHANG Jun, LI Laihao, et al. Study on isolation and antioxidation activity of oligopeptides from Pinctada fucata muscle[J]. Sci Technol Food Ind, 2012, 32(7): 123-126. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg9zcGd5a2oyMDEyMDcwMzQaCHdidHlyODh3
    [13]
    李琳, 李槿年, 余为一. 细菌分类鉴定方法的研究概况[J]. 安徽农业科学, 2004, 32(3): 549-551. doi: 10.3969/j.issn.0517-6611.2004.03.088

    LI Lin, LI Jinnian, YU Weiyi. Research progress of technology for bacterial identification[J]. J Anhui Agric Sci, 2004, 32(3): 549-551. (in Chinese) doi: 10.3969/j.issn.0517-6611.2004.03.088
    [14]
    HUGENHOLTZ P, HUBER T. Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases[J]. Int J Syst Evol Microbiol, 2003, 53(1): 289-293. doi: 10.1099/ijs.0.02441-0
    [15]
    KONISLY J. Colicins and other bacteriocins with established modes of action[J]. Annu Rev Microbiol, 1982, 36: 125-144. doi: 10.1146/annurev.mi.36.100182.001013
    [16]
    WHITFORD M F, MCPHERSON M A, FORSTER R J. Identification of bacteriocin-like inhibitors from rumen Streptococcus spp. and isolation and characterization of bovicin 255[J]. Appl Environ Microbiol, 200l, 67(2): 569-574. doi: 10.1128/AEM.67.2.569-574.2001
    [17]
    TAGG J R, DAJANI A S, WANNAMAKER L W. Bacteriocins of gram-positive bacteria[J]. Bacteriol Rev, 1976, 40(3): 722-756. doi: 10.3920/BM2018.0126
    [18]
    王树杉, 于敏, 张晓华. 海洋细菌素的研究进展[J]. 海洋科学, 2012, 36(1): 125-131. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1oeWt4MjAxMjAxMDIxGghlYjE1aTdlYg%3D%3D

    WANG Shushan, YU Min, ZHANG Xiaohua. Research and prospect of bacteriocins from marine bacteria[J]. Mar Sci, 2012, 36(1): 125-131. (in Chinese) https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0Eg1oeWt4MjAxMjAxMDIxGghlYjE1aTdlYg%3D%3D
    [19]
    HWANHLEM N, BURADALENG S, WATTANACHANT S, et al. Isolation and screening of lactic acid bacteria from Thai traditional fermented fish (Plasom) and production of Plasom from selected strains[J]. Food Control, 2011, 22(3/4): 401-407. doi: 10.1016/j.foodcont.2010.09.010
    [20]
    JENSEN P R, FENICAL W. Strategies for the discovery of secondary metabolites from marine bacteria: ecological perspectives[J]. Annu Rev Microbiol, 1994, 48: 559-565. doi: 10.1146/annurev.mi.48.100194.003015 Abstract
    • Related Articles

  • Cited by

    Periodical cited type(4)

    1. 赖胜，杨慧林，陈美玲，龙馨怡，刘淑丽，简敏菲. 鄱阳湖南矶湿地苔草植被根际土壤微生物群落结构分析. 江西师范大学学报(自然科学版). 2023(01): 82-90 .
    2. 冯敬宾，任春华，江晓，严岩，董俊德，胡超群. 大亚湾夏季表层浮游细菌生物量分布与环境变量的关系. 海洋湖沼通报. 2019(02): 81-89 .
    3. 关统伟，向慧平，王鹏昊，邓奥宇，董丹，赵顺先，张习超. 基于高通量测序的郫县豆瓣不同发酵期细菌群落结构及其动态演替. 食品科学. 2018(04): 106-111 .
    4. 宋宇. 草莓不同生长时期土壤微生物群落结构变化. 贵州农业科学. 2018(08): 59-62 .

    Other cited types(3)

Catalog

    Get Citation
    PDF
    XML
    Article views (3634) PDF downloads (2206) Cited by(7)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return