Abstract: The calcium content in prawn meat was determined by using microwave digestion-flame atomic absorption spectrometry (FAAS), and the optimal conditions of microwave digestion and FAAS program were studied. The results show that the optimal amount of digestion reagent HNO₃ is 5.0 mL when the sampling amount of prawn meat is 0.500 g. The optimal microwave digestion program for calcium determination in prawn meat is multistep digestion process D, which includes 3 steps: 1) power 400 W, ratio 100%, heat-up time 5 min, digestion temperature 120 ℃ for 5 min; 2) power 800 W, ratio 100%, heat-up time 5 min, digestion temperature 150 ℃ for 10 min; 3) power 800 W, ratio 100%, heat-up time 10 min, and digestion temperature 180 ℃ for 10 min. The calcium content in microwave-digested solution remains stable within 48 h. Under those conditions, the relative standard deviation (RSD) of determination is 5.284%, and the recovery of calcium is 91%~98%. The microwave digestion-FAAS method is rapid, accurate, economical and safe in determining the calcium content in prawn meat.