Effects of different substrate environments on growth, shell color and antioxidant capacity of Babylonia areolata juveniles
-
摘要:
为优化方斑东风螺 (Babylonia areolata) 健康养殖技术，比较分析了方斑东风螺稚螺在5种底质环境下 (河砂、棕色陶粒、黑色陶粒、黄色陶粒、白色陶粒) 的生长、壳色及抗氧化性能差异。结果表明，河砂组中底质氨氮 (
${\rm{NH}}^{{\text{+}}}_4 $ -N) 质量浓度为 (0.382±0.010) mg·L−1，显著高于其他实验组 (P<0.05)；棕色陶粒组的成活率最高 [(62.00±2.00)%]，显著高于河砂 [(56.00±1.00)%]、黄色陶粒 [(48.00±2.00)%] 和白色陶粒 [(42.50±3.00)%] 组 (P<0.05)；河砂组和棕色陶粒组的壳高、壳宽、体质量显著高于黑色、黄色、白色陶粒组 (P<0.05)。实验结束时，棕色陶粒组的壳色更艳丽，其壳色的明度值 (L*)、红度值 (a*)、黄度值 (b*) 皆显著高于其他实验组 (P<0.05)。棕色陶粒组总抗氧化能力显著高于其他实验组，而过氧化氢酶活性和丙二醛质量摩尔浓度分别为 (12.614±0.378) U·mg−1和 (1.179±0.001) nmol·mg−1，均低于其他实验组。综上所述，底质环境会显著影响方斑东风螺稚螺的生长、壳色和抗氧化性能，其中棕色陶粒较适合方斑东风螺稚螺的养殖，在生产中可以选用棕色陶粒替代河砂作为底质。Abstract:To optimize the healthy culture technology of Babylonia areolata, we compared and analyzed its growth, shell color and antioxidant properties under five substrate environments (River sand, brown ceramsite, black ceramsite, yellow ceramsite, white ceramsite). The results indicat that the ammonia nitrogen content in the river sand group [(0.382±0.010) mg·L−1] was significantly higher than that in the other groups (P<0.05). The highest survival rate was observed in brown ceramide group [(62.00±2.00)%], which was significantly higher than that of river sand group [(56.00±1.00)%], yellow ceramides group [(48.00±2.00)%] and white ceramides group [(42.50±3.00)%] (P<0.05). The shell height, shell width and body mass in river sand group were significantly higher than those in black, yellow and white ceramides groups (P<0.05). At the end of the experiment, the values of lightness, redness and yellowness of shell color in brown ceramide group were significantly higher than those in the other groups (P<0.05). The total antioxidant capacity (T-AOC) in brown ceramide group was significantly higher than that in the other groups (P<0.05). However, the catalase (CAT) activity and malonaldehyde (MDA) content were lower than those in the other groups, with (12.614±0.378) U·mg−1 and (1.179±0.001) nmol·mg−1, respectively. In conclusion, substrate environment can significantly affect the growth, shell color, and antioxidant performance of juvenile B. areolate, and brown ceramide can be selected to replace river sand as the substrate in its farming.
-
Keywords:
- Babylonia areolata /
- Juvenile snail /
- Sediment /
- Shell color /
- Antioxidant performance
-
南沙海域位于北纬3°40′~12°00′，东经108°30′~118°00′，面积约82万平方公里，有230多个岛礁分布其中，是我国神圣疆域不可分割的一个部分。南沙海域地处交通要冲，自然资源丰富，不仅是我国渔民传统的作业渔场，更是建设21世纪海上丝绸之路的战略基地，在我国海洋强国战略和国民经济发展体系中占据无可替代的重要地位。
近年来，围绕南海主权尤其是南沙岛礁主权的争端日趋频繁，六国七方纷争，局面复杂，我国南海主权维护面临严峻态势。目前南海渔业依然是我国南海维权最现实的重要手段，具有“屯海戍边”的战略意义。为了践行党中央、国务院关于“开发南沙，渔业先行”战略决策，充分发挥科技对南海现代渔业的引领和支撑作用，在农业部、财政部的大力支持下，“南海渔业资源调查与评估”项目(以下简称“南锋”专项)于2012年底正式立项，由中国水产科学研究院南海水产研究所承担。项目的实施，将为南沙渔业开发、管理以及主权斗争提供科学依据和技术支撑。
2013年，“南锋”专项针对南沙海域开展了4个航次的系统调查，累计海上调 查138天，航行2.1万海里，调查范围覆盖了南 沙群岛及邻近逾80万平方公里的海域，最远处到达了北纬3°58′的曾母暗沙，这不仅是建国以来南沙海域历次渔业调查中范围最广、时间最长、手段最先进的一次大规模调查，更是新形势下落实国家持续推进南海战略、切实加强海洋维权的一次重要科考，取得了丰硕的工作成果。
本期“南沙群岛及邻近海域综合调查研究专刊”是对“南锋”专项阶段性成果之总结，共收录18篇学术论文，内容涵盖南沙海域渔业资源、生态环境、捕捞技术、高值化加工及渔业管理等多个学科的最新研究成果，将为今后南沙渔业资源的合理开发利用、生态环境保护等提供重要的基础资料。
中国水产科学研究院南海水产研究所所长 “南海渔业资源调查与评估”项目总协调人 江世贵 2015年8月 -
![]()
图 1 方斑东风螺外壳形态指标测量模式图
Figure 1. Measurement of shell morphology indicators of juvenile B. areolata
![]()
图 2 不同底质中氨氮和亚硝酸盐质量浓度
注：不同字母代表不同养殖底质各实验组之间存在显著性差异 (P<0.05)，下同。
Figure 2. Mass concentrations of NH4 +-N and NO2 −-N in different substrate groups
Note: Different letters indicate significant differences among different groups (P<0.05). The same below.
![]()
图 3 不同底质养殖的方斑东风螺稚螺成活率
Figure 3. Survival rate of juvenile B. areolata in different substrate groups
![]()
图 4 不同底质养殖的方斑东风螺稚螺生长差异
Figure 4. Growth differences of juvenile B. areolata in different substrate groups
![]()
图 5 不同底质养殖的方斑东风螺稚螺贝壳的明度、红度和黄度
Figure 5. Lightness, redness and yellowness of juvenile B. areolata in different substrate groups
-
[1] 沈铭辉. 东风螺早期发育的生物学研究[D]. 厦门: 厦门大学, 2016: 1. [2] FU J Q, SHEN M H, SHEN Y W, et al. LC-MS/MS-Based metabolome analysis of biochemical pathways altered by food limitation in larvae of ivory shell, Babylonia areolata[J]. Mar Biotechnol (NY), 2018, 20(4): 451-466. doi: 10.1007/s10126-018-9808-6
[3] LYU W G, ZHONG M C, FU J Q, et al. Comparison and optimal prediction of goptimal prediction of growth of Babylonia areolata and B. lutosa[J]. Aquac Rep, 2020, 18: 100425. doi: 10.1016/j.aqrep.2020.100425
[4] 朱丽丹, 王晓清, 曾志南, 等. 3个群体方斑东风螺线粒体COI基因的遗传多样性分析[J]. 湖南农业大学学报(自然科学版), 2016, 42(4): 429-434. [5] 翁文明, 蔡岩, 卢明辉, 等. 方斑东风螺工厂化半循环水养殖试验[J]. 科学养鱼, 2021(3): 64-65. [6] 董杨, 符书源, 王国福. 海南省方斑东风螺养殖业的发展及其前景展望[J]. 科学养鱼, 2011(12): 39-40. [7] MACIEL C R, VALENTI W C. Effect of tank colour on larval performance of the Amazon River prawn, Macrobrachium amazonicum[J]. Aquac Res, 2014, 45(6): 1041-1050. doi: 10.1111/are.12048
[8] 凌慧. 方斑东风螺消化道和养殖环境细菌数量及细菌多样性研究[D]. 上海: 上海海洋大学, 2017: 4. [9] MACFARLANE G R, BOOTH D J. Estuarine macrobenthic community structure in the hawkesbury river, Australia: relationships with sediment physicochemical and anthropogenic parameters[J]. Environ Monit Assess, 2001, 72: 51-78. doi: 10.1023/A:1011959721146
[10] SIVADAS S, INGOLE B, NANAJKAR M. Temporal variability of macrofauna from a disturbed habital in Zuari estuary, west coast of India[J]. Environl Monit Assess, 2011, 173(7): 65-78.
[11] 潘英, 陈锋华, 李斌, 等. 管角螺对几种环境因子的耐受性试验[J]. 水产科学, 2008, 27(11): 566-569. [12] 何进金, 韦信敏, 许章程. 缢蛏稚贝饵料和底质的研究[J]. 水产学报, 1986(1): 29-39. [13] 吴志广, 聂顺泽. 不同养殖因素对青蛤生长影响的试验报告[J]. 河北渔业, 2002(3): 30. [14] 杨章武, 郑雅友, 李正良, 等. 方斑东风螺水泥池养殖不同底质的生长与存活试验[J]. 福建水产, 2011, 33(2): 29-32. [15] SHI C, WANG J C, PENG K W, et al. The effect of tank colour on background preference, survival and development of larval swimming crab Portunus trituberculatus[J]. Aquaculture, 2019, 504: 454-461. doi: 10.1016/j.aquaculture.2019.01.032
[16] TAMAZOUZT L, CHATAIN B, FONTAINE P. Tank wall colour and light level affect growth and survival of Eurasian perch larvae (Perca fluviatilis L.)[J]. Aquaculture, 2000, 182(1): 85-90.
[17] 廖秀睿, 杨金灵, 魏淼, 等. 不同养殖水色对红螯螯虾稚虾存活、生长和体色的影响[J]. 南方水产科学, 2022, 18(1): 77-83. [18] DUARTE R C, STEVENS M, FLORES A A. Shape, colour plasticity, and habitat use indicate morph-specific camouflage strategies in a marine shrimp[J]. BMC Evol Biol, 2016, 16(1): 1-15. doi: 10.1186/s12862-015-0575-y
[19] SIEGENTHALER A, MASTIN A, DUFAUT C, et al. Background matching in the brown shrimp Crangon crangon: adaptive camouflage and behavioural-plasticity[J]. Sci Rep, 2018, 8(1): 3292. doi: 10.1038/s41598-018-21412-y
[20] 钟继承, 范成新. 底泥疏浚效果及环境效应研究进展[J]. 湖泊科学, 2007, 19(1): 1-10. [21] 彭明国, 葛秋凡, 李美娟, 等. 利用电镀污泥制备水处理用陶粒滤料[J]. 电镀与涂饰, 2018, 37(3): 142-146. [22] 贾兰, 童欢欢, 周继梅, 等. 新型功能性陶粒制备及水处理效能研究[J]. 东北农业大学学报, 2016, 47(10): 74-82. [23] 王师君. 鲟鱼微流水养殖渔业能耗测算与节能措施分析[J]. 山东畜牧兽医, 2022, 43(1): 47-49. [24] 赵珍. 单过硫酸氢钾对低换水养殖环境和对虾生长的影响[D]. 北京: 中国科学院大学 (中国科学院海洋研所), 2021: 3. [25] DUTRA F M, FORNECK S C, BRAZAO C C, et al. Acute toxicity of ammonia to various life stages of the Amazon river prawn, Macrobrachium amazonicum, Heller, 1862[J]. Aquaculture, 2016, 453: 104-109. doi: 10.1016/j.aquaculture.2015.11.038
[26] 邵长清, 高勤峰, 董双林, 等. 过碳酸钠对刺参养殖底质改良和生长效果的研究[J]. 海洋湖沼通报, 2018(4): 126-132. [27] 散剑娣, 蔡德所, 欧文昌, 等. 底泥陶粒的制备及其性能研究[J]. 金属矿山, 2022(11): 229-237. [28] 靖青秀, 张呈熙, 黄晓东, 等. 铜渣基水处理陶粒的制备及性能研究[J]. 功能材料, 2020, 51(8): 8100-8104. [29] ABED G R, ZENG C S. Effects of tank colour on larval survival and development of mud crab Scylla serrata (Forskal)[J]. Aquac Res, 2005, 36: 1112-1119. doi: 10.1111/j.1365-2109.2005.01328.x
[30] LECLERCQ E, TAYLOR J F, MIGAUD H. Morphological skin colour changes in teleosts[J]. Fish Fish (Oxf), 2010, 11(2): 159-193.
[31] SHEN Y W, ZHANG Y, XIAO Q Z, et al. Distinct metabolic shifts occur during the transition between normoxia and hypoxia in the hybrid and its maternal abalone[J]. Sci Total Environ, 2021, 794: 148698. doi: 10.1016/j.scitotenv.2021.148698
[32] YU F, WU Y Y, SHEN Y W, et al. Heat adhesion duration: a new high-throughput abalone thermal tolerance assessment method[J]. Aquaculture, 2021, 545: 737226. doi: 10.1016/j.aquaculture.2021.737226
[33] BISWAS A K, SEOKA M, TAKII K, et al. Stress response of red sea bream Pagrus major to acute handling and chronic photoperiod manipulation[J]. Aquaculture, 2006, 252(2): 566-572.
[34] POERTNER H O, FARRELL A P. Physiology and climate change[J]. Science, 2008, 322(5902): 690-692. doi: 10.1126/science.1163156
[35] 林志华, 王铁杆, 夏彩国. 管角螺生态及繁殖习性观察[J]. 海洋科学, 1998(5): 11-12. [36] ZHENG X, LIAO X R, ZHANG M, et al. The effect of aquarium color background on the survival, growth performance, body coloration, and enzymatic activity of laboratory cultured Cherax quadricarinatus juveniles[J]. Aquac Rep, 2023, 32: 101699.
[37] 甘洋. 方斑东风螺嗅觉及其对海洋酸化的响应[D]. 厦门: 厦门大学, 2017: 1-3. [38] 王平川, 张立斌, 潘洋, 等. 脉红螺摄食节律的研究[J]. 水产学报, 2013, 37(12): 1807-1814. [39] 张爱菊, 尤仲杰. Nassarius属织纹螺摄食行为及毒素的研究进展[J]. 水利渔业, 2006, 26(1): 9-12. [40] 张林林. 管角螺摄食、生长及能量收支研究[D]. 宁波: 宁波大学, 2012: 74. [41] 刘永, 梁飞龙, 毛勇, 等. 方斑东风螺的人工育苗高产技术[J]. 水产养殖, 2004(2): 22-25. [42] REGUNATHAN C, WESLEY S G. Pigment deficiency correction in shrimp broodstock using Spirulina as a carotenoid source[J]. Aquac Nutr, 2006, 12(6): 425-432. doi: 10.1111/j.1365-2095.2006.00444.x
[43] TUME R K, SIKES A L, TABRETT S, et al. Effect of background colour on the distribution of astaxanthin in black tiger prawn (Penaeus monodon): effective method for improvement of cooked colour[J]. Aquaculture, 2009, 296(1/2): 129-135.
[44] LI K, CAI C F, YE Y T, et al. Comparison of non-volatile compounds and sensory characteristics of Chinese mitten crabs (Eriocheir sinensis) reared in lakes and ponds: potential environmental factors[J]. Aquaculture, 2012, 364-365: 96-102. doi: 10.1016/j.aquaculture.2012.08.008
[45] 张跃环, 闫喜武, 张澎, 等. 贝类壳色多态的研究概况及展望[J]. 水产科学, 2008, 27(12): 680-683. [46] CHRISTIANSEN R, Estermann R, TORRISSEN O J, et al. Assessment of flesh colour in Atlantic salmon, Salmo salar L.[J]. Aquac Res, 1995, 26(5): 311-321. doi: 10.1111/j.1365-2109.1995.tb00919.x
[47] 张春艳, 张震, 仇钧仪, 等. 饲料添加复合氨基酸对锦鲤生长和生理生化指标的影响[J]. 经济动物学报, 2022, 26(4): 261-267. [48] MANRIQUEZ P H, LAGOS N A, JARA M E, et al. Adaptive shell color plasticity during the early ontogeny of an intertidal keystone snail[J]. P Nat Acad Sci USA, 2009, 106(38): 16298-16303. doi: 10.1073/pnas.0908655106
[49] STEVENS M, RONG C P, TODD P A. Colour change and camouflage in the horned ghost crab Ocypode ceratophthalmus[J]. Biol J Linn Soc Lond, 2013, 109(2): 257-270. doi: 10.1111/bij.12039
[50] 段丽菊, 王晓平, 严彦, 等. NO在甲醛介导的氧化损伤中的协同作用[J]. 环境科学, 2006, 26(3): 505-508. [51] MARTINEZ A R M, MORALES A E, SANZ A. Antioxidant defenses in fish: biotic and abiotic factors[J]. Rev Fish Biol Fish, 2005, 15(1/2): 75-88.
[52] 叶建生, 王兴强, 马甡, 等. 盐度突变对凡纳滨对虾非特异性免疫因子的影响[J]. 海洋水产研究, 2008, 29(1): 38-43. [53] 樊甄姣, 刘志鸿, 杨爱国. 氨氮对栉孔扇贝血淋巴活性氧含量和抗氧化酶活性的影响[J]. 海洋水产研究, 2005, 26(1): 23-27. [54] MATHEW S, KUMAR K A, ANANDAN R, et al. Changes in tissue defence system in white spot syndrome virus (WSSV) in-fected Penaeus monodon[J]. Comp Biochem Physiol C, 2007, 145(3): 315-320.
[55] 魏淼, 顾志峰, 潘志, 等. 背景色对红螯螯虾生长、存活、体色及栖息行为的影响[J]. 海洋科学, 2020, 44(10): 60-65. [56] 马湘成, 张高立, 高广春, 等. 养殖容器颜色对拟穴青蟹幼蟹生长、应激和甲壳颜色的影响[J]. 宁波大学学报(理工版), 2021, 6(34): 43-49. [57] KANG D Y, KIM H C. Influence of density and background color to stress response, appetite, growth, and blind-side hypermelanosis of flounder, Paralichthys olivaceus[J]. Fish Physiol Biochem, 2013, 39(2): 221-232. doi: 10.1007/s10695-012-9693-2
下载:
计量
- 文章访问数: 391
- HTML全文浏览量: 124
- PDF下载量: 91
粤公网安备 44010502001741号
