鲮原种群体的AFLP分析

AFLP analysis on original population of mud carp Cirrhinus molitorella

摘要: 运用AFLP技术，对广东鲮鱼原种场保存的鲮原种2个子群体（子群体h、q）各29个个体进行遗传多样性分析。25对AFLP引物组合中的6对引物扩增共产生173条条带，其中多态位点数为72，多态位点比例为41.5%。在6对引物产生的173条扩增条带中，有一条扩增条带在子群体h中的出现频率远远高于其在子群体q中的出现频率（72.4%20.6%），编号为E2M4.1。遗传多样性分析结果表明，子群体h的遗传多样性指数高于子群体q（0.13670.0998）。该研究为筛选鲮原种群体2个子群体间的特异性分子标记做了初步尝试。

Abstract: Amplified fragment length polymorphism （AFLP） technique was applied to assess the genetic variations of the original population collected from Original Species Field of Cirrhinus molitorella in Guangdong Province. The studied samples contained sub-stock h and sub-stock q, 29 individuals respectively. Amplifications with 6 pairs of primers gave 173 reproducible and stable fragments, of which 72 were polymorphic. There was one band whose frenquency was much higher in sub-stock h than that was in sub-stock q （72.4%20.6%）. We named it as E2M4-1. Based on AFLP data, we found that the genetic diversity of sub-stock h was higher than sub-stock q （0.13670.0998）. This study is a preliminary attempt for special molecular marker selecting of the two wild sub-stocks.