重组鲮IGF-I对鲮GH表达的影响

The effect of recombinant mud carp insulinlike growth factor-Ⅰ(IGF-Ⅰ） on expression of growth hormone（GH）in mud carp，Cirrhinus molitorella

摘要: 使用半定量RT-PCR方法检测了鲮各组织中GH mRNA的表达，并分析重组鲮IGF-Ⅰ处理后鲮GH表达变化情况。结果表明：鲮的GH mRNA表达具有明显的组织特异性，在鲮的肝、肾、脾、肌肉、肠、鳃、性腺、心脏和皮肤中都未检测到GH mRNA表达，只在鲮脑中检测到GH mRNA表达；经重组鲮IGF-Ⅰ处理后，鲮脑中GH mRNA稍有升高，但在肝和肌肉中仍未检测到GH mRNA表达；重组鲮IGF-Ⅰ处理前后，鲮血清中GH浓度变化不明显。

Abstract: The study was conducted to examine the expression of GH mRNA in different tissues of mud carp by semi-quantitative RT-PCR and the effects of recombinant mud carp insulin-like growth factor-Ⅰ(rmcIGF-Ⅰ） on expression of GH in mud carp. GH mRNA detection result of brain of mud carp was positive, and the other tissues detections results were negative, including liver, kidney, spleen, muscle, intestine, gill, gonad, heart and skin. GH mRNA levels increased a little in brain in response to rmcIGF-Ⅰ, but the treated fish exhibited no obvious change in sera GH concentration following rmcIGF-Ⅰ injection.