Abstract: The mode of segregation of random amplified polymorphic DNA (RAPD) markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1:1 segregating loci account for 43.8% and 3:1 segregating loci 56.2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%，15.6%和56.2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1:1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.