高权新, 谢明媚, 彭士明, 张晨捷, 施兆鸿. 急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响[J]. 南方水产科学, 2016, 12(2): 59-66. DOI: 10.3969/j.issn.2095-0780.2016.02.009
引用本文: 高权新, 谢明媚, 彭士明, 张晨捷, 施兆鸿. 急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响[J]. 南方水产科学, 2016, 12(2): 59-66. DOI: 10.3969/j.issn.2095-0780.2016.02.009
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GAO Quanxin, XIE Mingmei, PENG Shiming, ZHANG Chenjie, SHI Zhaohong. Effect of acute temperature stress on metabolic enzymes, ion enzymes and concentration of ion in serum of juvenile Pampus argenteus[J]. South China Fisheries Science, 2016, 12(2): 59-66. DOI: 10.3969/j.issn.2095-0780.2016.02.009
Citation: GAO Quanxin, XIE Mingmei, PENG Shiming, ZHANG Chenjie, SHI Zhaohong. Effect of acute temperature stress on metabolic enzymes, ion enzymes and concentration of ion in serum of juvenile Pampus argenteus[J]. South China Fisheries Science, 2016, 12(2): 59-66. DOI: 10.3969/j.issn.2095-0780.2016.02.009
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急性温度胁迫对银鲳幼鱼代谢酶、离子酶活性及血清离子浓度的影响

Effect of acute temperature stress on metabolic enzymes, ion enzymes and concentration of ion in serum of juvenile Pampus argenteus

    摘要
  • 摘要: 为探明急性温度胁迫对银鲳( Pampus argenteus) 幼鱼生化指标的影响, 该实验设计了3 个温度梯度, 即22 ℃、27 ℃和32 ℃, 以27 ℃为对照组, 胁迫时间48 h, 分别测定胁迫前后其代谢酶、离子酶活性以及血清中离子浓度。结果显示, 2 个实验组的血清谷丙转氨酶( GPT) 的变化趋势相反, 肝脏谷草转氨酶( GOT) 的变化趋势也不同; 2 个实验组的碱性磷酸酶( AKP) 变化趋势相反, 而酸性磷酸酶( ACP) 变化趋势相同, 乳酸脱氢酶( LDH) 在 22 ℃实验组出现波浪式变化( P 0. 05) ; 血清钠( Na+ ) 、钾( K+ ) 、氯( Cl- ) 、钙( Ca2+ ) 在各组呈现不同的变化趋势; 鳃Na+ /K+ -ATP酶活性在32 ℃实验组出现下降趋势, 肾脏Na+ /K+-ATP酶活性在22 ℃实验组出现先升高后降低趋势( P 0. 05) ; 2 个实验组鳃Ca2+/Mg2+-ATP酶活性和肾脏Ca2+/Mg2+-ATP酶活性皆出现相反的变化趋势。结果表明, 急性温度胁迫可显著影响银鲳幼鱼的正常代谢, 因此在养殖过程中应尽量避免温度胁迫, 以使其有一个良好的生长环境。

     

    Abstract: To investigate the effect of acute temperature stress on biochemical indicators of juvenile Pampus argenteus, we designed two treatment groups ( 22 ℃ and 32 ℃) and a control group ( 27 ℃) with stress time of 42 h. The GPT in serumand GOT in liver in two experimental groups changed oppositely; AKP varied in an opposite manner but ACP changed in the same way; LDH in 22 ℃ group changed in a wave-like manner ( P 0.05) . Na+ , K+ , Cl- and Ca2+ concentrations in three groups changed differently. The Na+ /K+-ATPase activity in gill in 32 ℃ group decreased, but that in kidney in 22 ℃ group increased first then decreased ( P 0. 05) . In two experimental groups, Ca2+ /Mg2+-ATPase activities in gill and kidney varied in oppositely ( P 0.05) . It is suggested that acute temperature stress, which should be avoided in breeding for juvenile P. argenteus, affects normal metabolismof the juveniles significantly.

     

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