娄忠纬, 徐坤华, 王潇, 饶华俊, 戴志远. 鳙内源性转谷氨酰胺酶特性的研究[J]. 南方水产科学, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
引用本文: 娄忠纬, 徐坤华, 王潇, 饶华俊, 戴志远. 鳙内源性转谷氨酰胺酶特性的研究[J]. 南方水产科学, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
shu
LOU Zhongwei, XU Kunhua, WANG Xiao, RAO Huajun, DAI Zhiyuan. Characteristics of transglutaminase from bighead carp[J]. South China Fisheries Science, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
Citation: LOU Zhongwei, XU Kunhua, WANG Xiao, RAO Huajun, DAI Zhiyuan. Characteristics of transglutaminase from bighead carp[J]. South China Fisheries Science, 2014, 10(6): 72-77. DOI: 10.3969/j.issn.2095-0780.2014.06.010
shu

鳙内源性转谷氨酰胺酶特性的研究

Characteristics of transglutaminase from bighead carp

    摘要
  • 摘要: 文章研究了温度、pH、二硫苏糖醇（DTT）以及不同的金属离子和抑制剂对鳙（Hypophthalmichthys nobilis）鱼肉中内源性转谷氨酰胺酶（transglutaminase，TGase）活性的影响。结果表明，鳙内源性TGase的最适温度在37~45 ℃之间，最适pH为6.5~7.5，适量的钙离子（Ca2+）（0~2.2 mmolL-1）可以激发酶活性，低浓度（0~0.2 mmolL-1）的DTT可以提高酶活性，但高浓度的钠离子（Na+）和钾离子（K+）会降低酶活性，镁离子（Mg2+）、铜离子（Cu2+）、锌离子（Zn2+）、钡离子（Ba2+）、铁离子（Fe3+）、亚铁离子（Fe2+）、铅离子（Pb2+）和乙二胺四乙酸（EDTA）也会抑制酶的活性。

     

    Abstract: We studied the transglutaminase (TGase) from bighead carp (Hypophthalmichthys nobilis) under different conditions (temperature, pH, DTT). The optimum temperature and pH of TGase were 37~45 ℃ and 6.5~7.5, respectively. The optimum concentrations of CaCl2 and dithiothreitol were 0~2.2 mmolL-1 and 0~0.2mmolL-1, respectively. The TGase activity decreased as concentrations of Na+ and K+ increased, and was inactivated by Mg2+, Cu2+，Zn2+, Ba2+，Fe3+，Fe2+，Pb2+ and EDTA.

     

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