魏大鹏, 单洪伟, 马甡, 张家松. 混料设计优化复合菌剂比例的研究[J]. 南方水产科学, 2014, 10(1): 86-91. DOI: 10.3969/j.issn.2095-0780.2014.01.013
引用本文: 魏大鹏, 单洪伟, 马甡, 张家松. 混料设计优化复合菌剂比例的研究[J]. 南方水产科学, 2014, 10(1): 86-91. DOI: 10.3969/j.issn.2095-0780.2014.01.013
shu
WEI Dapeng, SHAN Hongwei, MA Shen, ZHANG Jiasong. Optimization of formula of compound bacteria preparation using mixture design[J]. South China Fisheries Science, 2014, 10(1): 86-91. DOI: 10.3969/j.issn.2095-0780.2014.01.013
Citation: WEI Dapeng, SHAN Hongwei, MA Shen, ZHANG Jiasong. Optimization of formula of compound bacteria preparation using mixture design[J]. South China Fisheries Science, 2014, 10(1): 86-91. DOI: 10.3969/j.issn.2095-0780.2014.01.013
shu

混料设计优化复合菌剂比例的研究

Optimization of formula of compound bacteria preparation using mixture design

    摘要
  • 摘要: 采用混料设计（Mixture）对复合菌比例进行优化，测定了3株芽孢杆菌(Bacillus spp.)和1株溶藻弧菌（Vibrio alginolyticus）不同比例搭配对细菌生长的光密度（OD）、氨氮和亚硝氮去除率的影响，建立了各搭配比例与试验指标之间的回归方程。使用Design Expert 8.0的优化功能（Optimization）对满足所有期望的响应值进行优化，得到复合菌的最优比例为芽孢杆菌BD6株 5.2%、BZ5株22%、溶藻弧菌VZ5 株72.8%。对该最优比例进行验证试验，得到的结果与预测基本一致。按上述比例搭配的复合菌株在第24 小时即可获得较高的氨氮和亚硝氮去除率，第72 小时的氨氮去除率为98.37%、亚硝氮去除率为93.81%。混料设计和调优软件可用于混合菌中多组分和多目标参数优化，以获得最优的复合菌比例组合。

     

    Abstract: Using mixture design to optimize formula of mixed bacteria, we studied the effects of 4 strains of bacteria on OD600, ammonia and nitrite removal rate, and establisheda regression model. Through the equation analysis and optimization of Design Expert 8.0, the response values satisfying all expectations were optimized, and the most excellent combinations of Bacillus sp. BD6, Bacillus sp. BZ5 and Vibrio alginolyticus VZ5were 5.2%, 22% and 72.8%, respectively. The result of verification experiment on the formulationwas consistent with the prediction. Ammonia and nitrite removal rate by the mixed bacteria was higherat 24th hour, our reaching 98.37% and 93.81% at 72th hour, respectively. Mixture design and analysis software can optimize the formulation of mixed bacteria for several responses and obtain the best formulation.

     

    • HTML全文
    • 参考文献(1)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回