朱佳杰, 李莉萍, 唐瞻杨, 周宇, 罗永巨, 甘西. 吉富罗非鱼家系构建及抗病力检测[J]. 南方水产科学, 2012, 8(6): 22-27. DOI: 10.3969/j.issn.2095-0780.2012.06.004
引用本文: 朱佳杰, 李莉萍, 唐瞻杨, 周宇, 罗永巨, 甘西. 吉富罗非鱼家系构建及抗病力检测[J]. 南方水产科学, 2012, 8(6): 22-27. DOI: 10.3969/j.issn.2095-0780.2012.06.004
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ZHU Jiajie, LI Liping, TANG Zhanyang, ZHOU Yu, LUO Yongju, GAN Xi. Family establishment and disease resistance of different families of GIFT Nile tilapia Oreochromis niloticus[J]. South China Fisheries Science, 2012, 8(6): 22-27. DOI: 10.3969/j.issn.2095-0780.2012.06.004
Citation: ZHU Jiajie, LI Liping, TANG Zhanyang, ZHOU Yu, LUO Yongju, GAN Xi. Family establishment and disease resistance of different families of GIFT Nile tilapia Oreochromis niloticus[J]. South China Fisheries Science, 2012, 8(6): 22-27. DOI: 10.3969/j.issn.2095-0780.2012.06.004
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吉富罗非鱼家系构建及抗病力检测

Family establishment and disease resistance of different families of GIFT Nile tilapia Oreochromis niloticus

    摘要
  • 摘要: 文章以吉富罗非鱼（GIFT strain Oreochromis niloticus）为研究对象，按照1雄配1雌原则进行家系配对，待家系鱼生长至50~60 g尾-1时人工感染无乳链球菌（Streptococcus agalactiae）（菌株GD001）。通过对GD001菌株半数致死浓度测定及各家系感染死亡率的统计，研究了GD001无乳链球菌感染对各家系吉富罗非鱼抗病力的影响。结果表明，1）配对成功家系67个，经繁殖性能筛选后留种53个，家系留种率为79.1%；2）GD001菌株的半数致死浓度为4108 cfumL-1，感染后2~6 d为死亡高峰期；3）GD001菌感染53个家系后有11个家系的成活率在90%以上，15个家系的成活率在70%~89%，19个家系的成活率在30%~69%，8个家系的成活率低于30%，表明不同家系对GD001菌株的抗病力存在着显著的差异（P0.05）。

     

    Abstract: The families of GIFT strain Nile tilapia (Oreochromis niloticus) were established by pair-mating and the juveniles (50~60 g) in each family were challenged by Streptococcus agalactiae (GD001 strain). According to the median lethal concentration (LC50) and infection mortality rate of each family, we evaluated the disease resistance of GIFT strain Nile tilapia to the challenge of S.agalactiae GD001 strain. The results show that: 1) 67 full-sibling families were successfully established, and 53 families (79.1%) were maintained based on their reproductive performance; 2) The LC50 of S.agalactiae GD001 strain to tilapia was 4108 cfumL-1; 3) Among the 53 families, 11 families had survival rate of over 90%, 15 families (70%~89%), 19 families (30%~69%) and 8 families (less than 30%). It is suggested that the disease resistancesof different GIFT families challenged with S.agalactiae were significantly different (P0.05).

     

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