壳寡糖对凡纳滨对虾生长和免疫力的影响

李振达; 陈小娥; 廖智; 蒋雅美

doi:10.3969/j.issn.2095-0780.2011.04.006

壳寡糖对凡纳滨对虾生长和免疫力的影响

Effects of chitooligosaccharides on growth and immunity of Litopemaeus vannamei

摘要

摘要: 为研究壳寡糖（chitooligosaccharides，COS）对凡纳滨对虾（Litopemaeus vannamei）生长和非特异性免疫水平的影响,设置了含COS 分别为0(对照)、250 mgkg-1、500 mgkg-1、750 mgkg-1和1 000 mgkg-1的5组试验饲料，进行了4周的饲养试验。结果显示，COS添加量为250 mgkg-1和500 mgkg-1时凡纳滨对虾血细胞总数、生长率和成活率显著性提高（P0.05），含COS 250 mgkg-1时对虾血清中的过氧化物酶（peroxidase，POD）活力显著提高（P0.05），添加量为500 mgkg-1时酸性磷酸酶（acid phosphatase，ACP）和溶菌酶（lysozyme，LSZ）活力显著提高（P0.05），添加量为250 mgkg-1和500 mgkg-1时总超氧化物歧化酶（total superoxide dismutase，T-SOD）活力提高不显著（P0.05）。由此表明饲料中添加250~500 mgkg-1 COS可使凡纳滨对虾细胞免疫和体液免疫因子总体活力达到较高水平，可显著提高凡纳滨对虾的生长率和成活率。

Abstract: A 4-week feeding trial was conducted to investigate the effects of chitooligosaccharides (0, 250 mgkg-1, 500 mgkg-1, 750 mgkg-1 and 1 000 mgkg-1) on the growth and non-specific immunization of Litopenaeus vannamei. Results demonstrate that there is significant difference in the total number of hemocytes, growth rate and survival of L.vannamei at 250 and 500 mg?kg-1 COS (P0.05); there is significant difference in peroxidase activity of L.vannamei at 250 mgkg-1 COS (P0.05); the acid phosphatase activity and lysozyme activity of L.vannamei at 500 mgkg-1 COS are significantly improved (P0.05); there is no significant difference in the total superoxide dismutase activity at 250 mgkg-1and 500 mgkg-1 COS (P0.05). In conclusion, 250~500 mgkg-1 COS can improve the cell immunity and total activity of humoral immune factor, increasing the growth rate and survival of L.vannamei.

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