Abstract: A 56-day feeding trial on pompano (Trachinotus ovatus) was carried out to study the effects of dietary inositol onits growth, feed utilization and blood biochemical index. Diets with 5 levels of inositol (350 mgkg-1, 458 mgkg-1, 507 mgkg-1, 720 mgkg-1 and 1 050 mgkg-1) were fed to pompano at initial weight of (8.30 0.11) g, respectively. Weight gain (WG) and special growth rate (SGR) of 720 mgkg-1 group are significantly higher (P0.05) than those of the other groups. Viscerasomatic index (VSI) is not significantly (P0.05) affected by inositol. Survival of 458 mgkg-1, 507 mgkg-1 and 720 mgkg-1 groups are significantly higher than that of 350mg.kg-1 and 1 050 mgkg-1 groups(P0.05). The moisture and lipid contentof 507 mgkg-1 groupare significantly higher and lower than those of the other groups except the 720 mgkg-1 one (P0.05), respectively.No significant difference is found in crude protein content (P0.05). The highest glucose (GLU) content is found in 720 mgkg-1 group, which is significantly higher than that of 350 mgkg-1 and 458 mgkg-1 groups (P0.05). Low density lipoprotein cholesterol (LDCL) of 507 mgkg-1 group is significantly lower than that of the other groups except 1 050 mgkg-1 one (P0.05). Supplementation of dietary inositol can significantly decrease triacylglycerol (TG) content but has no effect on total protein (TP), blood urea nitrogen (BUN), total cholesterol (TC) and high density lipoprotein cholesterol (HDLC).