马国强, 高天翔, 孙典荣. 基于16S rRNA 和Cyt b 基因序列探讨2 种梅童鱼的遗传分化[J]. 南方水产科学, 2010, 6(2): 13-20. DOI: 10.3969/j.issn.1673-2227.2010.02.003
引用本文: 马国强, 高天翔, 孙典荣. 基于16S rRNA 和Cyt b 基因序列探讨2 种梅童鱼的遗传分化[J]. 南方水产科学, 2010, 6(2): 13-20. DOI: 10.3969/j.issn.1673-2227.2010.02.003
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MA Guoqiang, GAO Tianxiang, SUN Dianrong. Discussion of relationship between Collichthys lucidus and C.niveatus based on 16S rRNA and Cyt b gene sequences[J]. South China Fisheries Science, 2010, 6(2): 13-20. DOI: 10.3969/j.issn.1673-2227.2010.02.003
Citation: MA Guoqiang, GAO Tianxiang, SUN Dianrong. Discussion of relationship between Collichthys lucidus and C.niveatus based on 16S rRNA and Cyt b gene sequences[J]. South China Fisheries Science, 2010, 6(2): 13-20. DOI: 10.3969/j.issn.1673-2227.2010.02.003
shu

基于16S rRNA 和Cyt b 基因序列探讨2 种梅童鱼的遗传分化

Discussion of relationship between Collichthys lucidus and C.niveatus based on 16S rRNA and Cyt b gene sequences

    摘要
  • 摘要: 比较分析了棘头梅童鱼（Collichthys lucidus）和黑鳃梅童鱼（C.niveatus）的16S rRNA和Cyt b基因片段序列差异及遗传分化程度。在长度为526 bp的16S rRNA和379 bp的Cyt b基因片段的核苷酸序列中，2种间共检测到44处核苷酸替代。分析结果表明,2个基因片段的鸟嘌呤（G）含量较低，在Cyt b蛋白质编码基因第三密码子位点上表现尤为明显。基于16S rRNA和Cyt b基因片段分析结果显示，2种间平均遗传距离分别为0.012和0.111。构建的系统树显示2种梅童鱼在这2个基因片段上存在显著的遗传分化。根据Cyt b基因2％/百万年的进化速率推断,棘头梅童鱼与黑鳃梅童鱼的分化时间约为550万年，发生于上新世（Pliocene）早期。

     

    Abstract: Collichthys lucidus and C.niveatus are commercially important fishery resources in China. Partial nucleotide sequences of mitochondrial DNA 16S rRNA and Cyt b gene fragments were sequenced and analyzed to study the relationship between C.lucidus and C.niveatus. Forty-four nucleotide substitutions were detected among 16S rRNA (526 bp) and Cyt b (379 bp) fragments. Nucleotide composition analysis indicated that the content of guanine (G) was relatively low in the two fragments, especially at the third codon position of Cyt b genes. Average genetic distances based on 16S rRNA and Cyt b between C.lucidus and C.niveatus were 0.012 and 0.111, respectively. The phylogenetic tree showedthat there was significant genetic divergence between C.lucidus and C.niveatus. According to the evolutionary rate of 2% perone million years, the estimated divergence time between C.lucidus and C.niveatus was 5.5 million years, which indicated an early Pliocene divergence for them.

     

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