马斌, 苏航, 徐永江, 崔爱君, 姜燕, 颜寒, 冯元, 公衍军, 冯德军. 褐藻寡糖对花鲈幼鱼生长和生理指标及肠道组织形态的影响[J]. 南方水产科学. DOI: 10.12131/20240022
引用本文: 马斌, 苏航, 徐永江, 崔爱君, 姜燕, 颜寒, 冯元, 公衍军, 冯德军. 褐藻寡糖对花鲈幼鱼生长和生理指标及肠道组织形态的影响[J]. 南方水产科学. DOI: 10.12131/20240022
MA Bin, SU Hang, XU Yongjiang, CUI Aijun, JIANG Yan, YAN Han, FENG Yuan, GONG Yanjun, FENG Dejun. Effects of alginate oligosaccharide on growth performance, physiological indicators and intestinal morphology of Lateolabrax maculatus juvenile[J]. South China Fisheries Science. DOI: 10.12131/20240022
Citation: MA Bin, SU Hang, XU Yongjiang, CUI Aijun, JIANG Yan, YAN Han, FENG Yuan, GONG Yanjun, FENG Dejun. Effects of alginate oligosaccharide on growth performance, physiological indicators and intestinal morphology of Lateolabrax maculatus juvenile[J]. South China Fisheries Science. DOI: 10.12131/20240022

褐藻寡糖对花鲈幼鱼生长和生理指标及肠道组织形态的影响

Effects of alginate oligosaccharide on growth performance, physiological indicators and intestinal morphology of Lateolabrax maculatus juvenile

  • 摘要: 在饲料中添加低聚糖类益生元可有效提升鱼类的生长和免疫相关性能。褐藻寡糖是一类从海洋褐藻胶中提取的低聚糖类益生元,对水产养殖动物生长和免疫性能改善具有重要作用。为检验褐藻寡糖在花鲈 (Lateolabrax maculatus) 幼鱼生长与免疫性能方面的应用效果,在基础饲料中按照0、50、100和200 mg·kg−1添加褐藻寡糖配置实验用饲料,开展为期42 d的养殖实验。结果显示,褐藻寡糖添加量为100 mg·kg−1时,花鲈幼鱼的体质量增长率 (WGR) 和特定生长率 (SGR) 均最高,分别为 (96.50±7.95)% 和 (1.73±0.09)%·d−1,且能显著上调生长相关基因 (gh) 的表达 (P<0.05);同时,幼鱼肠道肌层厚度、绒毛高度和宽度显著优于对照组 (P<0.05),胰蛋白酶 (TRY)、淀粉酶 (AMS) 和脂肪酶 (LPS) 活性高于对照组。随着褐藻寡糖添加量的增加,花鲈幼鱼肝脏超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSH-Px) 和溶菌酶 (LZM) 活性均显著升高 (P<0.05),丙二醛 (MDA) 含量显著降低 (P<0.05)。可见,添加褐藻寡糖能显著提升花鲈幼鱼的机体免疫水平,并对其生长有一定的促进作用。研究结果可为制定褐藻寡糖促进花鲈健康生长的使用策略提供理论参考。

     

    Abstract: Addition of oligosaccharide prebiotics into feeds can effectively enhance growth and immune-related performance of fish. Alginate oligosaccharide, an oligosaccharide prebiotic extracted from marine fucoidan, plays an important role in improving growth and immune performance of aquaculture animals. In order to investigate the effects of alginate oligosaccharide on the growth and immune performance of Lateolabrax maculatus juvenile, we conducted a 42-day experiment by adding alginate oligosaccharide to basic feed at doses of 0, 50, 100, and 200 mg·kg−1 (alginate oligosaccharide: basic feed) to prepare experimental feed. The results show that the highest weight gain rate (WGR) (96.50±7.95)% and specific growth rate (SGR) (1.73±0.09)%·d−1 of juvenile were observed with alginate oligosaccharide addition of 100 mg·kg−1. Expression of gh was significantly up-regulated in 100 mg·kg−1 group (P<0.05). Meanwhile, the intestinal muscular thickness, villus height, villus width of juveniles, as well as the activities of trypsin (TRY), amylase (AMS) and lipase (LPS) in 100 mg·kg−1 group were still higher than those of the control group (P<0.05). With increase of alginate oligosaccharide content, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and lysozyme (LZM) all increased significantly in liver of juvenile (P<0.05), while the malondialdehyde (MDA) content decreased significantly (P<0.05). Therefore, alginate oligosaccharide can improve the immune level of L. maculatus juvenile significantly. Besides, it has a certain promotion effect on its growth. The study provides theoretical references for the use of alginate oligosaccharide to promote the healthy growth of L. maculatus.

     

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