朱轩仪, 郑晓婷, 邢逸夫, 黄建华, 董宏标, 张家松. 三丁酸甘油酯能提高凡纳滨对虾鳃组织抗周期性高温胁迫能力[J]. 南方水产科学. DOI: 10.12131/20230246
引用本文: 朱轩仪, 郑晓婷, 邢逸夫, 黄建华, 董宏标, 张家松. 三丁酸甘油酯能提高凡纳滨对虾鳃组织抗周期性高温胁迫能力[J]. 南方水产科学. DOI: 10.12131/20230246
ZHU Xuanyi, ZHENG Xiaoting, XING Yifu, HUANG Jianhua, DONG Hongbiao, ZHANG Jiasong. Tributyrin improves resistance of gill tissues to periodic high-temperature stress for Litopenaeus vannamei[J]. South China Fisheries Science. DOI: 10.12131/20230246
Citation: ZHU Xuanyi, ZHENG Xiaoting, XING Yifu, HUANG Jianhua, DONG Hongbiao, ZHANG Jiasong. Tributyrin improves resistance of gill tissues to periodic high-temperature stress for Litopenaeus vannamei[J]. South China Fisheries Science. DOI: 10.12131/20230246

三丁酸甘油酯能提高凡纳滨对虾鳃组织抗周期性高温胁迫能力

Tributyrin improves resistance of gill tissues to periodic high-temperature stress for Litopenaeus vannamei

  • 摘要: 在水产养殖中,周期性高温 (Periodic high-temperature, PHT) 对虾类的生物学功能产生显著影响。鳃作为虾类的呼吸和代谢器官,在维持体内渗透平衡方面具有关键作用,但其功能容易受到高温等环境变化的干扰。为了探究有效的对虾PHT应对策略,研究了三丁酸甘油酯 (Tributyrin, TB) 对PHT (34±1) ℃, 7 d 胁迫下凡纳滨对虾 (Litopenaeus vannamei) 鳃组织形态和生理功能的影响。结果表明,PHT胁迫导致了显著的鳃组织损伤,诱导了氧化应激反应,进而造成内质网应激、细胞凋亡以及渗透调节功能障碍。不同饲喂策略下 (胁迫前饲喂三丁酸甘油酯7 d,每天饲喂三丁酸甘油酯,胁迫后饲喂三丁酸甘油酯7 d),鳃组织损伤程度均显著减轻,同时氧化应激水平降低。此外,三丁酸甘油酯还正向调节了PHT诱导的氧化应激、内质网应激、细胞凋亡以及渗透调节基因的相对表达水平。尤其是每天饲喂三丁酸甘油酯能够有效保持鳃组织形态完整,生理功能稳定。研究结果为对虾养殖中的环境应激应对策略提供了重要参考。

     

    Abstract: Periodic high-temperature (PHT) has a significant effect on biological function of shrimps in aquaculture. Gill, as the respiratory and metabolic organ of shrimp, plays a key role in maintaining the osmotic balance in fish body. However, its function is easily disturbed by environmental changes such as high temperature. In order to investigate effective strategies against periodic high-temperature in shrimp, we investigated the effects of tributyrin (TB) on the morphology and physiological function of gill tissue of Litopenaeus vannamei under PHT (34±1) ℃, 7 d stress. The results show that PHT stress caused significant gill tissue damage and induced oxidative stress, which in turn caused endoplasmic reticulum stress, apoptosis and osmotic regulation dysfunction. With different feeding strategies (Feeding tributyrin for 7 d before stress, feeding tributyrin every day including 7 stressed days, and feeding tributyrin for 7 d after stress), the degree of gill tissue damage significantly decreased, so did the level of oxidative stress. In addition, tributyrin positively regulated the relative expression levels of oxidative stress, endoplasmic reticulum stress, apoptosis and osmoregulation genes which were induced by PHT. In particular, daily feeding tributyrin was effective in maintaining the morphological integrity of gill tissues and stable physiological functions. The study provides important references for the strategies against environmental stress in shrimp aquaculture.

     

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