李敏, 叶毅飞, 李永福, 谢海燕, 李鸿超, 李春枝, 李希国, 李本旺, 陈灼均, 莫介化, 卢伟华, 朱燕秋, 张险朋. 黄唇鱼迟缓爱德华氏菌分离鉴定及药敏分析[J]. 南方水产科学. DOI: 10.12131/20230228
引用本文: 李敏, 叶毅飞, 李永福, 谢海燕, 李鸿超, 李春枝, 李希国, 李本旺, 陈灼均, 莫介化, 卢伟华, 朱燕秋, 张险朋. 黄唇鱼迟缓爱德华氏菌分离鉴定及药敏分析[J]. 南方水产科学. DOI: 10.12131/20230228
LI Min, YE Yifei, LI Yongfu, XIE Haiyan, LI Hongchao, LI Chunzhi, LI Xiguo, LI Benwang, CHEN Zhuojun, MO Jiehua, LU Weihua, ZHU Yanqiu, ZHANG Xianpeng. Isolation, identification and antimicrobial susceptibility test of Edwardsiella tarda in Bahaba taipingensis[J]. South China Fisheries Science. DOI: 10.12131/20230228
Citation: LI Min, YE Yifei, LI Yongfu, XIE Haiyan, LI Hongchao, LI Chunzhi, LI Xiguo, LI Benwang, CHEN Zhuojun, MO Jiehua, LU Weihua, ZHU Yanqiu, ZHANG Xianpeng. Isolation, identification and antimicrobial susceptibility test of Edwardsiella tarda in Bahaba taipingensis[J]. South China Fisheries Science. DOI: 10.12131/20230228

黄唇鱼迟缓爱德华氏菌分离鉴定及药敏分析

Isolation, identification and antimicrobial susceptibility test of Edwardsiella tarda in Bahaba taipingensis

  • 摘要: 2023 年入夏以来,东莞市黄唇鱼 (Bahaba taipingensis) 自然保护区救护基地出现黄唇鱼发病和死亡病例,症状主要为食欲下降,体表发红、充血,眼球突出,角膜白浊溃烂,吻部溃烂;解剖后发现腹腔有大量腹水,肝肿大呈褐色。为确定其病因,针对性制定防治措施,采集死亡病鱼的心脏、肝脏、脾脏、肾脏、肠、鳃丝等组织样品。从病死鱼体内分离到一株优势菌株,命名为DG230920,通过对菌株的形态特征、生理生化特性和16S rRNA基因序列综合分析,鉴定该菌为迟缓爱德华氏菌 (Edwardsiella tarda)。病理组织学检测结果显示,心脏、肝脏、脾脏、肾脏和鳃均有明显的炎症反应,肠见出血、黏膜上皮细胞脱落,脾淋巴细胞稀疏、内皮细胞变性坏死,肾小管变性坏死,鳃见鳃丝脱落、出血。对分离菌株进行药敏分析,结果为对环丙沙星、左氧氟沙星高度敏感,最低抑菌质量浓度仅≤0.25 mg·mL−1;其次是厄他培南,最低抑菌质量浓度≤0.5 mg·mL−1。结果表明,黄唇鱼发病原因可能是迟缓爱德华氏菌感染,分离菌株遗传序列稳定,对临床使用的抗菌药物均为敏感。研究提示,应加强黄唇鱼迟缓爱德华氏菌感染的防治,选用敏感药物科学治疗。

     

    Abstract: Since the summer of 2023, there have been cases of outbreak and death in the Bahaba taipingensis Natural Conservation Area in Dongguan City. The symptoms mainly include loss of appetite, reddening and congestion of body surface, protruding eyeballs, opaque and ulcerated corneas, as well as ulceration in snout. Dissection revealed a large amount of ascites in abdominal cavity and enlarged liver with brown color. To determine the cause of the disease, and make prevention and control measures, we collected tissue samples such as heart, liver, spleen, kidney, intestine and gills from the dead fish. A dominant strain was isolated from and named as DG230920. Through a comprehensive analysis of its morphological characteristics, physiological and biochemical properties, in addition to the 16S rRNA gene sequence analysis, the bacteria were identified as Edwardsiella tarda. Pathological histological examination results show obvious inflammatory reactions in heart, liver, spleen, kidney and gills. Hemorrhage and desquamation of epithelial cells were observed in intestine; sparse lymphocytes and necrosis of endothelial cells were found in the spleen; tubular degeneration and necrosis were observed in the kidney. Gills showed shedding and hemorrhage of gill filaments. Drug sensitivity analysis of the isolated strain revealed high sensitivity to ciprofloxacin and levofloxacin, with a minimum inhibitory concentration of only ≤0.25 mg·mL−1. The next most effective drug was imipenem, with a minimum inhibitory concentration of ≤0.5 mg·mL−1. The results indicate that the cause of B. taipingensis disease might be an infection with E. tarda, and the isolated strain exhibited stable genetic sequences and sensitivity to clinically used antibiotics. The study suggests that prevention and control measures should be strengthened against E. tarda infection in B. taipingensis, and sensitive drugs should be used for treatment.

     

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