王小燕, 周胜杰, 汪迎港, 孙永跃, 李铭浩, 马振华. 海水酸化对黄鳍金枪鱼幼鱼抗氧化和免疫能力的影响[J]. 南方水产科学. DOI: 10.12131/20230216
引用本文: 王小燕, 周胜杰, 汪迎港, 孙永跃, 李铭浩, 马振华. 海水酸化对黄鳍金枪鱼幼鱼抗氧化和免疫能力的影响[J]. 南方水产科学. DOI: 10.12131/20230216
WANG Xiaoyan, ZHOU Shengjie, WANG Yinggang, SUN Yongyue, LI Minghao, MA Zhenhua. Effects of acidification stress on antioxidant and immunity in juvenile yellowfin tuna (Thunnus albacares)[J]. South China Fisheries Science. DOI: 10.12131/20230216
Citation: WANG Xiaoyan, ZHOU Shengjie, WANG Yinggang, SUN Yongyue, LI Minghao, MA Zhenhua. Effects of acidification stress on antioxidant and immunity in juvenile yellowfin tuna (Thunnus albacares)[J]. South China Fisheries Science. DOI: 10.12131/20230216

海水酸化对黄鳍金枪鱼幼鱼抗氧化和免疫能力的影响

Effects of acidification stress on antioxidant and immunity in juvenile yellowfin tuna (Thunnus albacares)

  • 摘要: 大气中过多的二氧化碳 (CO2) 被海水吸收后形成碳酸,增加了海水酸度。探究海水酸化对黄鳍金枪鱼 (Thunnus albacares) 幼鱼抗氧化和免疫能力的影响,以为海洋生态环境和金枪鱼野生种群保护提供参考。以平均体长 (18.21±1.09) cm、平均体质量 (354.98±149.77) g的黄鳍金枪鱼幼鱼为研究对象,设定pH梯度为8.1、7.6、7.1和6.6,采用静水停食法,测定肝、鳃、红肌和皮肤中的丙二醛 (MDA) 含量以及超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSH-Px)、过氧化物酶 (POD)、酸性磷酸酶 (ACP)、碱性磷酸酶 (AKP) 和溶菌酶 (LZM) 的活性。结果显示:48 h后,pH 6.6处理组的幼鱼出现死亡。除对照组外,鳃中SOD、CAT、POD和LZM的活性均高于其他组织 (P<0.05),SOD、CAT、POD的活性在pH 7.1时达到峰值,LZM的活性在pH 6.6时达到峰值;皮肤的GSH-Px活性高于其他组织 (P<0.05),在pH 7.1时达到峰值;肝的MDA含量累积最多,ACP活性最高 (P<0.05),前者在pH 6.6时达到峰值,后者在pH 8.1时达到峰值;红肌的AKP活性显著低于其他组织 (P<0.05),且在pH 7.1时达到最低。综上所述,黄鳍金枪鱼幼鱼在海水pH大于7.1时,免疫功能和抗氧化防御系统存在一定的抵抗力,pH小于7.1之后,部分免疫功能出现紊乱。

     

    Abstract: Excess carbon dioxide in atmosphere is absorbed by seawater and then form carbonic acid, which increases seawater acidity. To investigate the effects of seawater acidification on antioxidant and immunity in juvenile yellowfin tuna (Thunnus albacares), and to provide references for the protection of marine ecosystems and wild stocks of tuna, we took juvenile yellowfin tuna Average body length of (18.21±1.09) cm and average body mass of (354.98±149.77) g as research subjects, and set the pH gradients of 8.1, 7.6, 7.1 and 6.6 to determine the malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) activities. The results show that after 48 h, the juveniles in pH 6.6 group showed mortality. Except for the control group, the activities of SOD, CAT, POD and LZM in gills were higher (P<0.05) than those of the other tissues, with the activities of SOD, CAT and POD peaking at pH 7.1, and the LZM activity peaking at pH 6.6; the GSH-Px activity in skin was higher than that in the other tissues (P<0.05), peaking at pH 7.1. In the liver, the MDA content was the most accumulated and the ACP activity was the highest (P<0.05), with the former peaking at pH 6.6 and the latter peaking at pH 8.1; the AKP activity in red muscle was significantly lower than that in the other tissues (P<0.05), reaching its lowest level at pH 7.1. The results show that there is some resistance of immune function and antioxidant defense system in juvenile yellowfin tuna when seawater pH is above 7.1, but has partial immune dysfunction when pH is lower than 7.1.

     

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