李兵部, 王桂兴, 张晓彦, 刘玉峰, 何忠伟, 曹巍, 任建功, 任玉芹, 张祎桐, 伞利择, 王玉芬, 侯吉伦. 牙鲆mstn基因SNPs位点多态性与生长性状的关联分析[J]. 南方水产科学. DOI: 10.12131/20230165
引用本文: 李兵部, 王桂兴, 张晓彦, 刘玉峰, 何忠伟, 曹巍, 任建功, 任玉芹, 张祎桐, 伞利择, 王玉芬, 侯吉伦. 牙鲆mstn基因SNPs位点多态性与生长性状的关联分析[J]. 南方水产科学. DOI: 10.12131/20230165
LI Bingbu, WANG Guixing, ZHANG Xiaoyan, LIU Yufeng, HE Zhongwei, CAO Wei, REN Jiangong, REN Yuqin, ZHANG Yitong, SAN Lize, WANG Yufen, HOU Jilun. Association analysis of SNPs polymorphisms in mstn gene of Paralichthys olivaceus with growth traits[J]. South China Fisheries Science. DOI: 10.12131/20230165
Citation: LI Bingbu, WANG Guixing, ZHANG Xiaoyan, LIU Yufeng, HE Zhongwei, CAO Wei, REN Jiangong, REN Yuqin, ZHANG Yitong, SAN Lize, WANG Yufen, HOU Jilun. Association analysis of SNPs polymorphisms in mstn gene of Paralichthys olivaceus with growth traits[J]. South China Fisheries Science. DOI: 10.12131/20230165

牙鲆mstn基因SNPs位点多态性与生长性状的关联分析

Association analysis of SNPs polymorphisms in mstn gene of Paralichthys olivaceus with growth traits

  • 摘要: 为研究牙鲆 (Paralichthys olivaceus) 肌肉生长抑制基因 (mstn) 多态性,开发其分子辅助育种新标记,采用重测序方法对120尾牙鲆 (3个双克隆杂交家系:60 尾;3个雌核发育家系:60 尾) 的mstn基因进行单核苷酸多态性 (Single nucleotide polymorphism, SNP) 位点筛选,并对不同基因型个体的生长性状进行多重比较及SNP标记验证。结果显示,共检测到7个SNPs位点,均为颠换型突变;外显子区3个 (Exonl 1: G2063A; Exonl 3: C3883T, C4009A),为同义突变;内含子区3个 (Intron 1: A2444T; Intron 2: T3816C, A3832C);3'端非编码区1个 (3'UTR: C4564A)。SNPs与生长性状关联分析结果表明,A3832C和C4564A标记位点与牙鲆体质量、体长、体高等生长性状显著相关 (P<0.05),其中A3832C 位点AA基因型和C4564A位点AC基因型在生长上表现出优势,A3832C位点CC和C4564A位点AA、CC基因型在生长上表现出劣势,其他5个SNPs位点对牙鲆生长性状影响不显著 (P>0.05)。将A3832C和C4564A标记位点在牙鲆生长快速群体 (F: 60 尾) 和生长缓慢群体 (S: 60 尾) 中验证,验证结果与多重比对结果一致,表明2个SNPs位点具有较高的稳定性。综上所述,研究筛选出了牙鲆mstn基因中与生长性状显著相关的A3832C和C4564A两个标记位点,为牙鲆分子辅助育种提供了理论参考。

     

    Abstract: In order to study the polymorphism of mstn gene in Japanese flounder (Paralichthys olivaceus) and develop new markers for its molecular assisted breeding, we srceened 120 individuals of Japanese flounder (Three double clone hybrid families: 60, three Gynogenesis families: 60) mstn genes for single nucleotide polymorphisms (SNP), and conducted multiple comparisons and SNPs markers validation on the growth traits with different genotypes. The results detected seven SNPs loci, all of which were transposition type mutation; three Exon regions (Exonl 1: G2063A; Exonl 3: C3883T, C4009A) were synonymous mutations; three Intron regions (Intron I: A2444T; Intron II: T3816C, A3832C); and one non Coding region at 3' end (3' UTR: C4564A). The correlation analysis between SNPs and growth traits show that A3832C and C4564A were significantly correlated with growth traits such as body mass, body length and body height (P<0.05). Among them, A3832C AA genotype and C4564A AC genotype showed advantages in growth; A3832C CC, C4564A AA and CC genotype showed disadvantages in growth, while the other SNPs loci had no significant impact on growth traits (P>0.05). A3832C and C4564A markers were validated in the fast-growing population (F: 60) and slow-growing population (S: 60), and the validation results are consistent with the results of multiple alignments, indicating that the two SNPs loci have high stability. In summary, this study screened two marker loci (A3832C, C4564A) in the mstn gene of Japanese flounder, which are significantly related to growth traits, providing theoretical references for its molecular assisted breeding.

     

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