李豪华, 廖涛, 白婵, 邱亮, 鉏晓艳, 李海蓝, 陈丽萍, 熊光权, 王炬光. 运前密度和温度驯化对斑点叉尾鮰幼鱼模拟运输的影响[J]. 南方水产科学. DOI: 10.12131/20230154
引用本文: 李豪华, 廖涛, 白婵, 邱亮, 鉏晓艳, 李海蓝, 陈丽萍, 熊光权, 王炬光. 运前密度和温度驯化对斑点叉尾鮰幼鱼模拟运输的影响[J]. 南方水产科学. DOI: 10.12131/20230154
LI Haohua, LIAO Tao, BAI Chan, QIU Liang, ZU Xiaoyan, LI Hailan, CHEN Liping, XIONG Guangquan, WANG Juguang. Effects of pre-transport density and temperature domestication on simulated transport of juvenile Ictalurus punctatus[J]. South China Fisheries Science. DOI: 10.12131/20230154
Citation: LI Haohua, LIAO Tao, BAI Chan, QIU Liang, ZU Xiaoyan, LI Hailan, CHEN Liping, XIONG Guangquan, WANG Juguang. Effects of pre-transport density and temperature domestication on simulated transport of juvenile Ictalurus punctatus[J]. South China Fisheries Science. DOI: 10.12131/20230154

运前密度和温度驯化对斑点叉尾鮰幼鱼模拟运输的影响

Effects of pre-transport density and temperature domestication on simulated transport of juvenile Ictalurus punctatus

  • 摘要: 探究了不同运前驯化方式对斑点叉尾鮰 (Ictalurus punctatus) 幼鱼运输过程中水质、生化参数及组织结构的影响,为其健康运输提供参考依据。通过控制密度 (鱼水质量比分别为1∶2、1∶3、1∶4) 和温度 (14、19、24 ℃) 两个条件对其先后进行运前密集驯化和温度驯化,随后模拟运输18 h,并考察模拟运输过程中 (第0、第2、第6、第18小时) 和运后恢复24 h的水质 (氨氮、pH)、生化参数 葡萄糖 (Glu)、皮质醇 (Cor)、乳酸脱氢酶 (LDH)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、丙二醛 (MDA) 以及组织结构变化 (皮肤、肠道)。结果显示,运前驯化密度1∶4、温度19 ℃处理组的氨氮显著低于其他实验组,pH显著高于其他实验组 (P<0.05),Glu、Cor、LDH、CAT、SOD和MDA优于其他实验组,并且皮肤和肠道结构损伤程度最低。研究表明,运前驯化密度1∶4、温度19 ℃能够有效提高斑点叉尾鮰幼鱼有水运输过程中的抗应激能力。

     

    Abstract: To explore the effects of different pre-transport acclimation methods on water quality, biochemical parameters and tissue structure of juvenile Ictalurus punctatus during transportation, and to provide references for transportation of juvenile I. punctatus, we conducted pre-transport intensive acclimation and temperature acclimation. The pre-transport intensive acclimation involved fish-to-water ratios of 1∶2, 1∶3 and 1∶4, and the temperature acclimation was at 14, 19 and 24 ℃. The simulation transport lasted for 18 h, and we investigated the changes in water quality (Ammonia nitrogen, pH), biochemical parameters Glucose (Glu)、cortisol (Cor), lactate dehydrogenase (LDH), catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) as well as organizational structure (Skin and intestine) at different time (0th, 2nd, 6th, 18th hour) and 24-hour recovery after shipment. The results show that pre-transport intensive acclimation with a fish-to-water ratio of 1∶4 and a temperature of 19 ℃ had lower ammonia nitrogen levels than the other treatments (P<0.05). Glu, Cor, LDH, CAT, SOD and MDA levels were better than the other groups, and there was minimal damage to the skin and intestinal structures. In conclusion, pre-transport intensive acclimation with a fish-to-water ratio of 1∶4 and a temperature of 19 ℃ can improve anti-stress capacity effectively during transportation of juvenile I. punctatus.

     

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