许海鑫, 朱洪赓, 张家敏, 朱明, 王海华, 陈建华. 碳酸盐碱度对大鳞副泥鳅胚胎发育和仔鱼活力的影响[J]. 南方水产科学. DOI: 10.12131/20230149
引用本文: 许海鑫, 朱洪赓, 张家敏, 朱明, 王海华, 陈建华. 碳酸盐碱度对大鳞副泥鳅胚胎发育和仔鱼活力的影响[J]. 南方水产科学. DOI: 10.12131/20230149
XU Haixin, ZHU Honggeng, ZHANG Jiamin, ZHU Ming, WANG Haihua, CHEN Jianhua. Effect of carbonate alkalinity on embryonic development and larval vitality of Pramisgurnus dabryanus[J]. South China Fisheries Science. DOI: 10.12131/20230149
Citation: XU Haixin, ZHU Honggeng, ZHANG Jiamin, ZHU Ming, WANG Haihua, CHEN Jianhua. Effect of carbonate alkalinity on embryonic development and larval vitality of Pramisgurnus dabryanus[J]. South China Fisheries Science. DOI: 10.12131/20230149

碳酸盐碱度对大鳞副泥鳅胚胎发育和仔鱼活力的影响

Effect of carbonate alkalinity on embryonic development and larval vitality of Pramisgurnus dabryanus

  • 摘要: 为探究大鳞副泥鳅 (Paramisgurnus dabryanus) 胚胎及仔鱼对碳酸盐碱度的适应性,将发育至囊胚期的胚胎分别放置于碳酸盐碱度为0、10、20、30 和40 mmol·L−1的水体中培养,记录胚胎的孵化率、孵化时间,统计仔鱼的畸形率、成活率、特定成长率及卵黄囊吸收率,并测定初孵仔鱼的不投饵存活系数 (Survival activity index, SAI)。结果显示:1) 碳酸盐碱度为10 mmol·L−1时,胚胎孵化率最高,但与对照组差异不显著 (P>0.05),碳酸盐碱度在10~20 mmol·L−1时,孵化时间显著短于对照组 (P<0.05);2) 初孵仔鱼的畸形率随碳酸盐碱度的增加而逐渐升高,3日龄 (Days after hatching, dah) 仔鱼成活率则随碳酸盐碱度的增加逐渐降低;3) 初孵仔鱼全长随碳酸盐碱度的增加先下降后上升,而3 dah仔鱼全长则随碳酸盐碱度的增加先上升后下降,其特定生长率及卵黄囊吸收率均呈先升后降的趋势;4) 随着碳酸盐碱度的增加,仔鱼SAI先升后降,其中10 mmol·L−1组达到最大值。结果表明,碳酸盐碱度在0~10 mmol·L−1范围内适宜大鳞副泥鳅的胚胎发育及仔鱼生长。

     

    Abstract: To investigate the adaptation of the embryos and larvae of Paramisgurnus dabryanus to carbonate alkalinity, we cultured the embryos which had developed to the blastocyst stage in water at carbonate alkalinities of 0, 10, 20, 30 and 40 mmol·L−1, so as to investigate the hatching period, hatching rate, abnormality rate, survival rate, specific growth rate (SGR), yolk sac absorption and survival activity index (SAI). The results show that: 1) The embryo hatching rate was highest at carbonate alkalinity of 10 mmol·L−1 without significant difference compared with that of the control group (P>0.05). The hatching period was significantly shorter than that of the control group at carbonate alkalinities of 10 and 20 mmol·L−1 (P<0.05). 2) The abnormality rate of the newly hatched larvae ascended gradually with the increase of the carbonate alkalinity, while the survival rate of larvae of 3 dah (Days after hatching) gradually decreased. 3) The total length of newly hatched larvae decreased and then increased, while the total length of larvae of 3 dah increased and then decreased with the increase of carbonate alkalinity. The SGR and yolk sac absorption rate showed an increasing and then decreasing tendency. 4) The larval SAI first increased and then decreased with the increase of carbonate alkalinity and reached the maximum in the 10 mmol·L−1 group. It is indicated that the optimal carbonate alkalinity for the embryonic development and larval growth of P. dableanus is 0–10 mmol·L−1.

     

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