平鲷消化系统形态学、组织学及组织化学研究

Histological and histochemical study of digestive system of Rhabdosargus sarba

  • 摘要: 对平鲷(Rhabdosargus sarba)消化系统结构进行观察,采用HE和AB-PAS染色方法分别从组织学和组织化学方面进行研究。结果表明,平鲷口咽腔宽大,具不同类型发达齿;食道粗短,内表面分布纵行粘膜褶;胃分为贲门、胃盲囊和幽门,贲门胃小凹和胃腺最多,胃盲囊厚度最大;肠道具丰富粘膜褶;幽门与前肠交界处有幽门盲囊8~10个。肝小叶分界不明显,管状结构丰富。胰腺腺泡和胰岛分布于肝脏内大静脉周围,腺泡细胞染色深蓝色。根据AB-PAS染色结果将消化道粘液细胞分为4型,Ⅰ型为红色,Ⅱ型为蓝色,Ⅲ型为紫红色,Ⅳ型为蓝紫色。胃部可见极少量Ⅱ型及Ⅲ型粘液细胞,其余部位均以Ⅱ型粘液细胞为主。结果表明,粘液细胞消化系统不同部位的分布密度与对应部位消化功能相关。

     

    Abstract: The histological structure and types of mucous cells in the digestive tracts (i.e., tongue, esophagus, stomach and intestines) of Rhabdosargus sarba were observed and analyzed by using HE and AB-PAS (alcian blue and periodic acid schiff′s reation) conbined staining. Results of study in which slices were stained with HE and showed that R.sarba has a broad oropharyngeal cavity and different type of teeth. The stomach is divided to three parts which are cardiac stomach, stomach caeca and pyloric stomach. The cardiac stomach has the most gastric pit and gastric gland while stomach caeca has the thickest wall. There are abundant mucous membrane pleats in the whole intestine. R.sarba has 8 to 10 pyloric caecas which structure is similar to anterior intestine. The dividing lines of liver lobules are dim. There are plenty of tubular tissue in hepatopancreas. Pancreatic acini and pancreatic islets distribute around main veins, acinar cells being stained into navy blue are closely arranged. According to AB-PAS, 4 types of the mucous cells in the digestive tracts of R.sarba were observed: typeⅠwas red, typeⅡ was blue, type Ⅲ was purple reddish, and typeⅣ was blue purple. Only a small quantity of type Ⅱ and type Ⅲ mucous cells were found occasionally in stomach. Most of mucous cells in the other digestive tracts were of type Ⅲ and type Ⅳ. The density of the mucous cells in the intestine was related to the functions of the different segments in the digestive system.

     

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