Abstract: The experiment was conducted to determine the supplement of microbiological preparations Bacillus licheniformis (BL), Rhodopseudomonas capsulate (RC), Lactobacillus spp. (LB) on ammonia-N, nitrite-N, nitrate-N, chemical oxygen demand and active phosphate in industrialized shrimp culture. The experiments was composed of two teams and every team had two frequencies: control group, BL+RC added once per 7 days (G1), BL+RC added once per 3 days (G2), LB+BL added once per 7 days (G3) and LB+BL added once per 3 days (G4). The results showed that different microbiological preparations had different preponderance. At the aspect of reducing NH₄⁺, compared with control group, G1, G2 and G3 reduced 62%, 63% and 65%, respectively.In the first 21 days, G2 reduced 79% compared with control group. As for NO₂^-, compared with comparison, G1 reduced 46%. The best effect for controlling water quality was group G1. In addition, no groups resulted in significantly reduced PO₄^3-and COD. There is no striking difference between two frequencies in controlling the water quality. With the culture time increasing each index trended to increase.