Abstract: The effect of temperature on digestive enzymes activity of yellow fin black porgy (stomach, hepatopancreas and intestinal) was studied in this experiment. The results of specific activity of protease at different temperature (15,20,25,30,35,40,45,50,55,65℃) indicated that the optimum temperature for protease in yellow fin black porgy were 45℃ in stomach, 40℃ in hepatopancreas and 45℃ in intestinal canal; the optimum temperature for amylase were 40℃ in stomach, 35℃ in hepatopancreas and 30℃ in intestinal canal; the optimum temperature for lipase were 40℃ in stomach, 45℃ in hepatopancreas and 40℃ in intestinal canal. In range of 35 to 45℃, the activity of digestive enzymes (protease, amylase, lipase）in stomach, hepatopancreas and intestinal canal of yellow fin black porgy are in a higher level.