温度对黄鳍鲷主要消化酶活性的影响

Effect of temperature on the activity of major digestive enzymes in yellow fin black porgy (Sparus latus)

摘要: 研究了温度对黄鳍鲷肝、肠和胃3个部位蛋白酶、淀粉酶和脂肪酶比活的影响。实验设计了15、20、25、30、35、40、45、50、55、60、65℃等11个温度梯度, 结果表明: 肠蛋白酶和胃蛋白酶的最适温度为45℃, 而肝蛋白酶的最适温度为40℃; 胃淀粉酶的最适温度是40℃, 肝淀粉酶的最适温度是35℃, 而肠淀粉酶的最适温度是30℃; 肠脂肪酶和胃脂肪酶的最适温度是40℃, 肝脂肪酶的最适温度是45℃。从整体来看, 在35~45℃的范围内, 肝、肠和胃3部位的3种酶都有较高的活性。

Abstract: The effect of temperature on digestive enzymes activity of yellow fin black porgy (stomach, hepatopancreas and intestinal) was studied in this experiment. The results of specific activity of protease at different temperature (15, 20, 25, 30, 35, 40, 45, 50, 55, 65℃) indicated that the optimum temperature for protease in yellow fin black porgy were 45℃ in stomach, 40℃ in hepatopancreas and 45℃ in intestinal canal; the optimum temperature for amylase were 40℃ in stomach, 35℃ in hepatopancreas and 30℃ in intestinal canal; the optimum temperature for lipase were 40℃ in stomach, 45℃ in hepatopancreas and 40℃ in intestinal canal. In range of 35 to 45℃, the activity of digestive enzymes (protease, amylase, lipase)in stomach, hepatopancreas and intestinal canal of yellow fin black porgy are in a higher level.