金鱼线粒体DNA Cyt b基因序列分析及与鲫鱼亲缘关系探讨

Sequence analysis of mitochondrial cytochrome b gene of Carassius auratus var.and phylogenetic relationships of C. auratus var.and C. auratus

    摘要
  • 摘要: 通过对金鱼(Carassius auratus var.)的5个代表品种草金(grass goldfish)、红龙睛(red dragoneye goldfish)、鹤顶红(red-head wen goldfish)、水泡(blisters-eye goldfish)、黑寿(white-head oval goldfish)线粒体DNA细胞色素b基因全序列进行测定，结果发现金鱼Cyt b基因全序列1 141 bp，其中T、C、A、G 4种碱基含量分别为29.1%，27.8%，28.4%，14.5%，A+T的含量(57.8%)高于C+G的含量(42.2%)。草金、红龙睛、鹤顶红、水泡、黑寿Cyt b基因全序列一样。结果表明，细胞色素b基因在金鱼种内是相当保守的，草金、红龙睛、鹤顶红、水泡、黑寿起源于同一祖先。将鲫鱼和金鱼线粒体Cyt b基因序列比较分析，同源性达97.9%，应该还在同一种的水平上。

     

    Abstract: Mitochondrial cytochrome b DNAs of representative varieties of Carassius auratus var., viz. grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish and white-head oval goldfish were sequenced. 1 141 bp of mitochondril cytochrome b gene sequence has been obtained from C. auratus var. and the content of T, C, A, G of the gene were 29.1%, 27.8%, 28.4%, 14.5%, respectively.The sequences were same in those varieties of C. auratus var. So the results showed that Cyt b gene was conservative and indicated grass goldfish, red dragoneye goldfish, red-head wen goldfish, blisters-eye goldfish, white-head oval goldfish may be the same species. According to the analysis, C. auratus var. is relatively closer to C. auratus. Sequence analysis of mtDNA Cyt b gene of C. auratus var. and C. auratus which has 97.9% homology suggests it may be reasonable in a species.

     

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