Abstract: The activities of digestive enzymes in three alimentary organs of Lutjanus sebae were detected and compared. The results showed that the protease activity was intestine > stomach > hepatopancreas (P < 0.01);and the amylase activity was intestine > hepatopancreas > stomach (P < 0.01);and the lipase activity was intestine > hepatopancreas > stomach. The lipase activity difference between intestine and hepatopancreas or stomach was significant (P < 0.01), but the difference between hepatopancreas and stomach was not significant (P > 0.05). The digestive activities of intestine in young L.sebae were high. The stomach and hepatopancreas were auxiliary organs.