Abstract: The chromosome specimens of high-finned grouper Cromileptes altivelis were obtained from metaphase of kidney cells by vivo injection of PHA and colchicin, hypato-air drying technique, as well as Giemsa staining. The results showed that C.altivelis had a diploid chromosome number of 48 and its karyotype formula was 2n=48=48t, NF=48. Its karyotype was in accordance with the typical characters of higher location karyotype group of fish, but yet a relatively primitive one in Perciformes.