菲律宾蛤仔2个壳色品系群体杂交的研究

Study on population hybridization of two shell color strains of Manila clam Ruditapes philippinarum

  • 摘要: 于2007年10月,以F1代海洋红(R)和斑马蛤(Z)为材料,开展了2个壳色菲律宾蛤仔(Ruditapes philippinarum)品系的群体杂交。试验由RR(R♀×R♂)、ZZ(Z♀×Z♂)、RZ(R♀×Z♂)和ZR(Z♀×R♂)组成。结果表明,2个壳色品系亲贝壳长、重量和产卵量差异显著(P<0.05)。各试验组卵径、受精率和D形幼虫大小无显著差异(P>0.05),但杂交组孵化率显著高于相应对照组(P<0.05)。浮游期间幼虫未表现出明显的生长优势,但表现出一定的存活优势。RZ和ZR的生长优势平均值分别为(1.63±0.81)%和(2.58±0.67)%;生长速度分别为(8.64±0.32)和(8.67±0.31)μm · d-1,显著高于相应对照组(P<0.05);存活优势分别为(10.30±1.92)%和(16.30±1.04)%。室内培育期间稚贝表现出明显的生长、存活优势。RZ、ZR的生长优势平均值分别为(11.25±2.98)%和(20.31±2.10)%;生长速度分别为(9.88±1.45)和(10.79±1.32)μm · d-1,显著高于相应对照组(P<0.05),存活优势分别为(40.85±9.90)%和(57.08±11.98)%。

     

    Abstract: The population hybridization was conducted by using clams of two shell color strains " ocean red" (R), " zebra" (Z) strains in October of 2007. The experiments was consisted of RR (R♀×R♂), ZZ (Z♀×Z♂), RZ(R♀×Z♂), ZR (Z♀×R♂) groups. The results showed that difference of shell length, weight and fecundity of parents were significant between two shell color strains (P < 0.05). There were no difference on egg-diameter, fertilized rate and size of D larvae among each experimental groups (P > 0.05).The hatching rates in hybrid groups were higher than in control groups (P < 0.05).At the planktonic stage, survival heterosis was obvious instead of growth heterosis for larvae tested. Survival heterosis were(10.30±1.92)%and(16.30±1.04)%, respectively for RZ and ZR, and growth heterosis for RZ, ZR were(1.63±0.81)%and(2.58±0.67)%.Growth rates for RZ, ZR were (8.64±0.32) and (8.67±0.31) μm · d-1, respectively (P < 0.05).At indoor rearing stage, obvious growth and survival heterosis were found. The growth heterosis of RZ, ZR were(11.25±2.98)%and(20.31±2.10)%, the growth rates were (9.88±1.45) and (10.79±1.32) μm · d-1, and the survival heterosis were(40.85±9.90)%and(57.08±11.98)%, respectively. There was no significant difference of survival heterosis between RR and ZZ (P < 0.05).

     

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