空间诱变钝顶螺旋藻优质高产品系的选育

The breeding of Spirulina platensis mutants with good quality and high yield induced by space flight

  • 摘要: 通过卫星搭载,利用空间环境诱变钝顶螺旋藻(Spirulina platensis)。从诱变材料中通过单株培养和继代培养选育出适合大规模生产的优质高产新品系PNK-2。与出发品系ST-6相比,PNK-2的螺旋个数为12~18个;藻丝体平均长度764.31 μm,增长了166.52%;螺距平均长度52.98μm,增长了5.88%;螺宽平均长度18.75 μm,增长了8.19%;藻丝宽平均长度为6.02 μm,增长了12.31%。室外大规模生产生产率提高了20%以上。藻粉蛋白质含量69.57%,提高了8.31%;叶绿素含量1.01%,提高了8.60%;β-胡萝卜素含量0.16%,提高了6.67%;藻蓝蛋白含量14.70%,提高了6.68%;γ-亚麻酸含量0.63%,减少3.08%。表明了PNK-2是一株优质高产的钝顶螺旋藻品系。

     

    Abstract: A Spirulina platensis mutant PNK-2 had been bred from S.platensis mutants which had been induced by space flight. PNK-2, with good quality and high yield, suits for out-door mass production. Comparing with the initial strain ST-6, the helix number of PNK-2 was 12~18, average length of algae body, thread pitch, helix width, diameter of trichome and the rate of large-scale production were 764.31, 52.98, 18.75, 6.02 μm and 10 g·(m2·d)-1, respectively; and the increase rate were 166.52%, 5.88%, 8.19%, 12.31% and 22.89%, respectively. The content of protein, chlorophyll, β-carotene and phycocyanin in PNK-2 were 69.57%, 1.01%, 0.16% and 14.70%, respectively; and the increase rate were 8.31%, 8.60%, 6.67% and 6.68%, respectively. The γ-linolenic acid content of PNK-2 was 0.63%, reducing 3.08%. The results showed that PNK-2 was a new S.platensis strain with good quality and high yield.

     

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