Abstract: To promote biomass of photosynthetic bacteria PS3 in industrial production, the study on cultivating parameters of PS3 was carried out. The cultivating parameters included: light source, light intensity, dissolved oxygen, cultivating media pH, salinity and inoculative concentration. The results showed that PS3 was eugenic under anaerobic condition, and the optimal inoculative concentration, light intensity, pH and salinity were 10%, 4 000 lx (tungsten lamp), 6.5~7.0 and 5, respectively.