自动定氮仪测定水产品中的挥发性盐基氮

Determination of total volatile basic nitrogen in aquatic products by nitrogen auto analyzer

摘要: 采用自动定氮仪测定法研究了罗非鱼(Tilapia niloticus)、蓝圆 (Decapterus maruadsi)、凡纳滨对虾(Litopenaeus vannamei)和近江牡蛎(Crassostrea rivularis)4种水产样品处理的高氯酸溶液浓度和浸提时间及其蒸馏前样液放置时间对挥发性盐基氮(TVBN)测定的影响。结果表明：以0.6 mol · L^-1高氯酸溶液均质样品2 min后测定4种水产品中TVBN，样品加标回收率在98.42%~100.18%之间，相对标准偏差在1.40%以下。其测定结果与GB/T 5009.44蒸馏法相比，不存在显著性差异，且操作简便、快速。

Abstract: Four species of aquatic products as Tilapia niloticus, Decapterus maruadsi, Litopenaeus vannamei and Crassostrea rivularis were pretreated by perchloric acid(HClO₄)solution to extract the total volatile basic nitrogen(TVBN), which was determined by nitrogen auto analyzer.The influence of different concentration of HClO₄ solution, extracting time and keeping time of the extract before distillation on determination was studied. The results showed that the recovery was between 98.42% and 101.18%, RSD was below 1.40%, by determinating the TVBN in aquatic products after the samples had been homogenized for 2 minutes at 0.6 mol · L^-1 HClO₄ solution.The result of this method had no significant difference compared with the distillation in GB/T 5009.44, and the operation was easy and rapid.