鳗弧菌注射对栉孔扇贝免疫活性的影响

Effects of Vibrio anguillarum on immune activities of Chlamys farreri

摘要: 测定了鳗弧菌(Vibrio anguillarum)注射对栉孔扇贝(Chlamys farreri)胞内活性氧(ROS)含量和过氧化氢酶(CAT)、酸性磷酸酶(ACP)和碱性磷酸酶(ALP)活性的影响。结果表明，在注射后5、24、48和72 h各免疫指标都有明显变化；胞内ROS含量在24、48和72 h明显升高，且均高于对照组，形成抛物线趋势，在48 h达到最高；血清中CAT活性均有升高趋势；肝脏中ACP活性在24、48和72 h与注射生理盐水组相比明显升高；栉孔扇贝体内ALP活性有明显升高的趋势，且在注射后24 h活性达到最高，而后有所下降。结果表明，鳗弧菌对栉孔扇贝免疫系统有明显的刺激作用。

Abstract: The content of intracellular ROS and the activities of CAT, ACP and ALP of Chlamys farreri were studied at 5, 24, 48 and 72 h after challenged with Vibrio anguillarum injection. The results showed that the intracellular ROS content of experimental groups were much higher than that of control groups at 24, 48, 72 h. The CAT activities of experimental groups in haemocytes were higher than that of control group but not significantly. The ACP and ALP activities of experimental groups were much higher than that of control group at 5, 24, 48, 72 h. In conclusion, V.anguillarum could influence the immune activities of C.farreri.