星突江鲽(Platichthys stellatus)群体同工酶分析

Isozyme analysis of a cultured Platichthys stellatus stock

  • 摘要: 采用水平凝胶电泳技术对星突江鲽(Platichthys stellatus)养殖群体进行了同工酶分析。筛选了9种酶,检测到15个位点,在0.99水平上,仅PGM*位点表现为多态,LDH*SDH*G3PDH*GPI-1*GPI-2*GPI-3*IDHP*SOD-1*SOD-2*SOD-3*MDH-1*MDH-2*MPI-1*MPI-2*均为单态,多态位点比例为6.67%,实际观测杂合度为0.0098,预期杂合度为0.0303,平均有效等位基因数目为1.0557。结果表明,星突江鲽养殖群体的遗传多样性水平较低。

     

    Abstract: Horizontal starch gel electrophoresis was used to investigate the genetic diversity of a cultured Platichthys stellatus stock. Only PGM* locus was polymorphic among fifteen loci which were examined from nine enzymes while the most common allele was less than or equal to 0.99. The proportion of polymorphic loci was 6.67%, the mean observed heterozygosity was 0.0089, and the mean expected heterozygosity was 0.0303. The average effective number of alleles was 1.0557.

     

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