金焰笛鲷rDNA基因转录间隔区ITS-1序列分析

Sequence analysis of the internal transcribed spacer 1(ITS-1) of ribosomal DNA gene of Lutjanus fulviflamma

摘要: 以特异性引物扩增了金焰笛鲷(Lutjanus fulviflamma)的核糖体第一转录间隔区(ITS-1)，扩增产物经克隆后测序，测得ITS-1长度为566 bp。其中A、T、G、C 4种碱基的含量分别为14.1%、16.1%、30.2%、39.6%，G+C(69.8%)含量明显高于A+T含量(30.2%)。将此引物在笛鲷属其他4种鱼类中扩增，发现该对引物有很好的通用性；比较发现在不同种中ITS-1存在着较大的差异，适合将其应用于分子系统学和种质资源方面的研究。

Abstract: The PCR technique was used to amplify rDNA-ITS-1 of Lutjanus fulviflamma, then the purified PCR productions were cloned into T-vector and sequenced by M13+/-primers.As a result, 566 bp nucleotide sequences of rDNA-ITS1 were obtained. The average contents of A, T, G and C were 14.1%, 16.1%, 30.2% and 39.6%, respectively, the contents of GC (69.8%) were obviously higher than those of AT. Using these primers, ITS-1 region can be amplified in other four Lutjanus species, but the lengths of ITS-1 were largely different between different species.