Abstract: A total of 284 strains (153 were isolated from intestines and 131 were isolated from farming water) were tested by Kirby-bauer diffusion method to study the resistance of Haliotis discus hannai in Fujian Province. Results show that many strains resisted penicillin G, kanamycin, gentamicin and rifampin. The resistant rates were up to 83.59%;the resistant rates to norfloxacin, chloramphenicol and enrofloxacin were all relatively low, and resistant rate to norfloxacin was the lowest in farming water. Thus, there is no different resistance between heterotrophic bacteria from intestines and farming water. Multiple antibiotic resistance (MAR) was widespread in different sources and the average MARs were 59.15% and 51.31% in intestines and farming water. The resistant rates to penicillin G, kanamycin, gentamicin and rifampin from intestines were the lowest in September and those from farming water were the lowest in June.