Abstract:
Cyclin T is a critical factor that stimulates the process of transcription elongation. We obtained the full-length cDNA sequence of
cyclin T from black tiger shrimps (
Penaeus monodon)(denoted as
Pmcyclin T) by RACE PCR. The cDNA of
Pmcyclin T was 3 421 bp, encoding a polypeptide of 1 044 amino acids. Bioinformatics analysis shows that the amino acid sequence contained a CYCLIN conserved region of a periodic protein family as well as N-glycosylation sites and phosphorylation sites. The results of Blast and phylogenetic analyses suggest that Pmcyclin T shared very high homology with other kinds of arthropod such as
Diaphorina citri,
Acromyrmex echinatior and so on. Besides, we measured the mRNA expression of
Pmcyclin T in seven tissues by real-time PCR. Expression was highest in the ovary and moderate in the intestine, brain and so on. While at different stages of ovary,
Pmcyclin T was highest expressed at Stage Ⅲ, suggesting that
Pmcyclin T might play an important role in ovary development of black tiger shrimp.