蜡样芽孢杆菌对对虾养殖水体微藻群落的调控研究

Regulation of Bacillus cereus to microalgae community in shrimp aquaculture water

  • 摘要: 运用实验生态学方法分析了溶藻细菌——蜡样芽孢杆菌(Bacillus cereus)CZBC1对对虾养殖水体微藻群落的影响。根据用菌量,实验分为J0组(不施菌)、J3组(用菌量3.00×103 CFU · mL-1)和J5组(用菌量3.00×105 CFU · mL-1),每组3个平行,各实验水体优势种绿色颤藻(Oscillatoria chlorine)和波吉卵囊藻(Oocystis borgei)的初始优势度为64.14%和16.42%,实验周期15 d。结果显示,经过15 d的实验,J0组绿色颤藻优势度显著高于J3组和J5组(P<0.01),波吉卵囊藻优势度显著低于J3组和J5组(P<0.01)。其中,J0组绿色颤藻优势度升高至81.86%,J3组和J5组则分别降低至16.7%和14.6%;J0组波吉卵囊藻优势度降低至3.13%,J3组和J5组则分别升高至32.42%和47.14%。J0组、J3组和J5组对虾死亡率在第6天时分别为82.2%、46.7%和31.1%,J0组显著高于J3组和J5组(P<0.01),第15天时J0组仍然高于J3组和J5组。可见,CZBC1可有效抑制绿色颤藻(蓝藻)的生长,从而为波吉卵囊藻(绿藻)占据生态优势提供有利空间,为养殖对虾提供良好的水生态环境。

     

    Abstract: We analyzed the effect of Bacillus cereus (algicidal bacteria CZBC1) on microalgae community in shrimp aquaculture water by method of experimental ecology. According to the algicidal bacteria amount,we set Group J0 (0 CFU · mL-1),Group J3 (3.0×103 CFU · mL-1) and Group J5 (3.0×105 CFU · mL-1) with three parallels. The initial dominances of Oscillatoria chlorine and Oocystis borgei,the dominant species in water,were 64.14% and 16.42%,respectively. The results show that the dominance of O.chlorine in Group J0 was significantly higher than those in Group J3 and Group J5 after 15-day culture (P<0.01),and their dominances were 81.86%,16.7% and 14.6%,respectively. While the dominance of O.borgei in Group J0 was significantly lower than those in Group J3 and Group J5 (P<0.01),and their dominances were 3.13%,32.42% and 47.14%,respectively. The mortality of shrimp in Group J0 was significantly higher than those in Group J3 and Group J5 on the 6th day (P<0.01),which were 82.2%,46.7% and 31.1%,respectively. Furthermore,the mortality in Group J0 was also higher than those in Group J3 and Group J5 on the 15th day. The results indicate that algicidal bacteria CZBC1 can inhibit the growth of O.chlorine (Cyanophyta) effectively,which helps occupy the ecological advantage for O.borgei (Chlorophyta),providing better water environment for the growth of shrimp.

     

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