Abstract: Taking chicken (Gallus gallus) red blood cells as internal reference standard, we measured the genome size of Nibea albiflora by using flow cytometry. The relative area and integrated optical density (IOD) of 24 pairs of chromosomes were determined using fluorescence staining and microscopic image analysis software, and the physical length of each pair of chromosomes was estimated based on the IOD values. The genome size of N.albiflora was estimated as (595.70±24.08) Mb, and the DNA content was (0.61±0.02) pg. The genome of N.albiflora comprised 24 pairs of telocentric chromosomes, with the physical length ranging from (16.85±4.13) Mb to (31.95±4.23) Mb. The physical lengths of chromosomes were correlated linearly with the relative area and relative lengths. The results provide basic data for analysis of evolution rule of genome size in Sciaenidae and the forthcoming whole genome sequencing of N.albiflora, as well as for identifying and paring of chromosomes.