Abstract: Vertebrae are usually used to determine age and growth for sharks. Recent studies have shown that vertebrae contain important information about feeding and trophic level. In this study, vertebrae of blue shark (Prionace glauca) were used to estimate stable isotope ratios of δ¹³C and δ¹⁵N which were -1.664‰~-1.308‰, 9.29‰~2.237‰, and 2.73~5.73, respectively. The vertebra samples were sub-sampled from the origin, middle and outer edge of centrum. The δ¹³C, δ¹⁵N, and TL between origin and middle, between middle and outer edge, and between origin and outer edge were not significantly different, indicating sub-sampling might be made at any of these positions. This results provide important information for further studies on trophic dynamics of pelagic sharks.