Abstract: The acute toxicities of mebendazole, deltamethrin and copper sulphate to Alosa sapidissima were studied by static water tests. At 15 ℃, 20 ℃ and 25 ℃, the 96 h median lethal concentrations (LC50) of mebendazole to A.sapidissima were 0.226 mLL-1, 0.077 mLL-1 and 0.122 mLL-1, and safe concentrations (SC) were 0.194 mLL-1, 0.027 mLL-1 and 0.063 mLL-1, respectively. The 96 h-LC50 of deltamethrin were 0.386 gL-1, 0.234 gL-1 and 0.259 gL-1, and SCs were 0.090 gL-1, 0.061 gL-1and 0.082 gL-1, respectively. The 96 h-LC50 of copper sulphate were 0.320 mLL-1, 0.385 mLL-1 and 0.186 mLL-1, and SCs were 0.042 mLL-1, 0.115 mLL-1 and 0.019 mLL-1, respectively. According to the standard of fish acute toxicity, mebendazole was high-toxic at 20 ℃ and was less toxic at 15 ℃ and 25 ℃. Deltamethrin and copper sulphate were high-toxic and middle-toxic at three water temperatures.The results reveal that temperature affected SCs of different drugs to A.sapidissima greatly. Thus, dose of fishery drugs should be adjusted cautiously according to different water temperatures.